| 货号 | 14167-100ug |
| 描述 | The BRCA1 C-terminal (BRCT) domain is a modular unit of ~100 amino acids that folds independently and recognizes linear phosphoserine or phosphothreonine regions to mediate protein-protein and protein-DNA interactions.1,2 BRCT domains were initially recognized in the C-terminal region of the breast cancer protein BRCA1, as well as the p53 binding protein (53BP1; TP53BP1), and the yeast cell cycle checkpoint protein RAD9.3 BRCT domains often occur as tandem repeats at the C-terminal end of several proteins that are functionally diverse.2 Most BRCT domain-containing proteins participate in DNA-damage checkpoint control or DNA-repair pathways, or both.4,3 Thus, BRCT domain-containing proteins likely participate in the cellular response to DNA damage.Germ-line mutations of the tumor suppressor genes BRCA1 and BRCA2 pre-dispose women to familial breast and ovarian cancer and are associated with approximately 10% of the cases.5,6 The carboxy-terminal BRCT domain in BRCA1 is involved in DNA repair and transcriptional activation.7,8,6 The BRCT region of BRCA1 binds phosphorylated BACH1, a DEAH helicase, which is essential for mediating repair of DNA double-strand breaks by controlling G2 to M phase transition during cell cycle progression.9,10,11 BRCA1 also contains an N-terminal protein-protein-interaction RING-finger domain, that partners with BRCA1-associated RING domain 1 (BARD1).12 The BRCA1/BARD1 heterodimer maintains genomic stability by regulating DNA damage repair and ubiquitination.13 This product contains the BRCT domains of BRCA1. |
| 别名 | Breast Cancer Type 1 Susceptibility Protein; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Manke, I.A.,Lowery, D.M.,Nguyen, A., et al. BRCT repeats as phosphopeptide-binding modules involved in protein targeting. Science 302(5645), 636-639 (2003). 2.Woods, N.T.,Mesquita, R.D.,Sweet, M., et al. Charting the landscape of tandem BRCT domain-mediated protein interactions. Science Signaling 5(242), rs6 (2012). 3.Bork, P.,Hofmann, K.,Bucher, P., et al. A superfamily of conserved domains in DNA damage-responsive cell cycle checkpoint proteins. The FASEB Journal 11(1), 68-76 (1997). 4.Callebaut, I. and Mornon, J.P. From BRCA1 to RAP1: A widespread BRCT module closely associated with DNA repair. FEBS Letters 400(1), 25-30 (1997). 5.ODonovan, P.J. and Livingston, D.M. BRCA1 and BRCA2: Breast/ovarian cancer susceptibility gene products and participants in DNA double-strand break repair. Carcinogenesis 31(6), 961-967 (2010). 6.Yarden, R.I. and Papa, M.Z. BRCA1 at the crossroad of multiple cellular pathways: Approaches for therapeutic interventions. Molecular Cancer Therapeutics 5(6), 1396-1404 (2006). 7.Metcalfe, K.A. and Narod, S.A. Breast cancer prevention in women with a BRCA1 or BRCA2 mutation. Open Med. 1(3), e184-e190 (2007). 8.Narod, S.A. Modifiers of risk of hereditary breast and ovarian cancer. Oncogene 25(43), 5832-5836 (2006). 9.Shiozaki, E.N.,Gu, L.,Yan, N., et al. Structure of the BRCT repeats of BRCA1 bound to a BACH1 phosphopeptide: Implications for signaling. Molecular Cell 14(3), 405-412 (2004). 10.Cantor, S.B.,Bell, D.W.,Ganesan, S., et al. BACH1, a novel helicase-like protein, interacts directly with BRCA1 and contributes to its DNA repair function. Cell 105(1), 149-160 (2001). 11.Yu, W.,Chini, C.C.S.,He, M., et al. The BRCT domain is a phospho-protein binding domain. Science 302(5645), 639-642 (2003). 12.Nishikawa, H.,Wu, W.,Koike, A., et al. BRCA1-associated protein 1 interferes with BRCA1/BARD1 RING heterodimer activity. Cancer Research 69(1), 111-119 (2009). 13.Brzovic, P.S.,Keeffe, J.R.,Nishikawa, H., et al. Binding and recognition in the assembly of an active BRCA1/BARD1 ubiquitin-ligase complex. Proceedings of the National Academy of Sciences of the United States of America 100(10), 5646-5651 (2003). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 纯度 | ≥90% (estimated by SDS-PAGE) |
| 稳定性 | ≥ 1 year |
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