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货号： bs-0095R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0095R
- 英文名称
- Calponin 1
- 中文名称
- 钙调节蛋白-1抗体
- 别 名
- Basic calponin; Calponin 1; Calponin-1; Calponin 1 basic smooth muscle; Calponin H1 smooth muscle; Calponin1; Calponins basic; CNN 1; CNN1; Sm Calp; SMCC; CNN1_HUMAN; Calponin H1; Calponin1; Sm Calp; smooth muscle.
- Specific References (4) | bs-0095R has been referenced in 4 publications.[IF=4.07] Syedain, Zeeshan, et al. ʺImplantation of Completely Biological Engineered Grafts Following Decellularization into the Sheep Femoral Artery.ʺ Tissue Engineering (2014). IHC-F ; Sheep.PubMed:24417686[IF=4.75] Zhao, Yuechao, et al. "Estrogen-Induced CCN1 is Critical for Establishment of Endometriosis-like Lesions in Mice." Molecular Endocrinology (2014). IHC-P ; Mouse.PubMed:25321413[IF=2.30] Cai, Jinghua, et al. "Regulation of tumorigenesis in oral epithelial cells by defined reprogramming factors Oct4 and Sox2." Oncology Reports IHC-P ; Mouse.PubMed:27279579[IF=1.83] HAYASHI, TAKUMA, et al. "Characterization of Leiomyomatoid Angiomatous Neuroendocrine Tumour (LANT)-like Tumour in the Myometrium with Histopathological Examination." Anticancer Research 37.4 (2017): 1765-1772. IHC ; Human.PubMed:28373439
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 心血管 信号转导 干细胞 细胞骨架
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 33kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Calponin 1:201-297/297
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Calponin, a calmodulin, is a thin filament associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. Calponin is expressed in smooth muscle and tissues containing significant amounts of smooth muscle. Two isoforms of calponin exist whose molecular weights are 34kDa and 29kDa. Expression of the 29kDa form is primarily restricted to muscle of the urogenital tract. The expression of calponin has also been demonstrated in myoepithelial cells from benign and malignant breast lesions.
Function:
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).
Subunit:
Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1.
Tissue Specificity:
Smooth muscle, and tissues containing significant amounts of smooth muscle.
Similarity:
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain.
SWISS:
P51911
Gene ID:
1264
Database links:Entrez Gene: 1264 Human
Entrez Gene: 484937 Dog
Entrez Gene: 12797 Mouse
Entrez Gene: 396911 Pig
Entrez Gene: 65204 Rat
Omim: 600806 Human
SwissProt: P51911 Human
SwissProt: Q08091 Mouse
SwissProt: Q08092 Pig
SwissProt: Q08290 Rat
Unigene: 465929 Human
Unigene: 4356 Mouse
Unigene: 31788 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Calponin1是一种激动蛋白结合蛋白，是平滑肌特有的一种蛋白，可以与肌动蛋白、原肌球蛋白和钙调素结合,具有抑制平滑肌细胞收缩的功能，主要用于平滑肌肿瘤和乳腺等组织中的肌上皮细胞分布的研究。
- 产品图片
Sample:
Urinary bladder (Mouse) Lysate at 40 ug
Primary: Anti-Calponin 1 (bs-0095R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Calponin 1 Polyclonal Antibody, Unconjugated(bs-0095R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Calponin 1 Polyclonal Antibody, Unconjugated(bs-0095R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Positive control: H9C2(2% Paraformaldehyde-fixed )
Isotype Control Antibody
Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA
Secondary Antibody
Antibody: Goat anti-rabbit IgG-FITC;Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody
Supplier catalog number: bs-0095R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA