| 货号 | 13101S |
| 使用方法 | NVP-BEZ235 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 1.065 ml DMF. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 5 nM-500 nM for 2-24 hr. |
| 供应商 | CST |
| 背景 | NVP-BEZ235 is a selective and potent dual inhibitor of PI3 kinase and mTOR Ser/Thr protein kinase. Research studies show that NVP-BEZ235 reversibly inhibits the catalytic activity of these kinases by competing with ATP at the ATP-binding site (1,2). Studies demonstrate that NVP-BEZ235 has anti-proliferative characteristics and induces cell cycle arrest during G0/G1 phase through inhibition of CDK4 and cyclin D3 (2,3). |
| 存放说明 | -20C |
| 纯度 | >99% |
| 计算分子量 | 469.54 g/mol |
| 参考文献 | 1 . Serra, V. et al. (2008) Cancer Res 68, 8022-30. 2 . Schult, C. et al. (2012) Anticancer Res 32, 463-74. 3 . Yang, S. et al. (2011) PLoS One 6, e26343. |
Chemical structure of NVP-BEZ235. | |
Western blot analysis of extracts from Jurkat cells, untreated or treated with NVP-BEZ235 (2 hr) at the indicated concentrations. The phosphorylation of p70 S6 kinase was detected using Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234 (upper) and p70 S6 Kinase (49D7) Rabbit mAb #2708 (lower). | |
Western blot analysis of extracts from Jurkat cells, untreated or treated with NVP-BEZ235 (2 hr) at the indicated concentrations. The phosphorylation of Akt was detected using Phospho-Akt (Ser473) (D9E) XP®Rabbit mAb #4060 (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). |
