| 货号 | 12819S |
| 使用方法 | Tunicamycin is supplied as a lyophilized powder. For a 5 mg/ml stock, reconstitute the 5 mg in 1 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-10 μg/ml for 0.5-20 hours. |
| 供应商 | CST |
| 背景 | Isolated from Streptomyces lysosuperificus, tunicamycin is a nucleoside antibiotic that inhibits N-linked glycosylation in glycoprotein synthesis. Composed of tunicamycins A, B, C and D, the compound competitively blocks the transfer of N-acetylglucosamine-1-phosphate (GlcNAc-1-P) from UDP-GlcNAc to dolichol-P (1,2). By preventing glycoprotein synthesis, tunicamycin inhibits the formation of the “viral coat” known as the tunica or capsid in both RNA and DNA viruses, and thus exhibits antiviral properties (3). Research studies have shown that tunicamycin will arrest cells in G1 phase, preventing them from entering S-phase and increasing the expression of PERK (4,5). Following N-linked glycosylation inhibition, tunicamycin induces autophagy in response to ER stress, ultimately upregulating CHOP and BIP (6,7). |
| 存放说明 | -20C |
| 纯度 | >99% |
| 计算分子量 | 840.9 g/mol |
| 参考文献 | 1 . Takatsuki, A. et al. (1971) J Antibiot (Tokyo) 24, 215-23. 2 . Harding, H.P. et al. (1999) Nature 397, 271-4. 3 . Kaushal, G.P. and Elbein, A.D. (1986) Plant Physiol 82, 748-52. 4 . Hirano, T. et al. (1982) J Biochem 92, 765-73. 5 . Brewer, J.W. and Diehl, J.A. (2000) Proc Natl Acad Sci U S A 97, 12625-30. 6 . Ding, W.X. et al. (2007) J Biol Chem 282, 4702-10. 7 . Zinszner, H. et al. (1998) Genes Dev 12, 982-95. |
Chemical structure of Tunicamycin. | |
Western blot analysis of C2C12 cell extracts, untreated (-) or treated with Tunicamycin (2 μg/ml, 8 hr; +), using CHOP (D46F1) Rabbit mAb #5554 (upper) or β-Actin Antibody #4967 (lower). |
