货号 | 12986S |
使用方法 | Epothilone B is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 100 µg in 197 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1000 nM for 12-48 hr. |
供应商 | CST |
背景 | Epothilone A and B are taxol-like macrolides originally identified as antifungal, cytotoxic metabolites derived from the myxobacterium Sorangium cellulosum. Research studies demonstrate that epothilone B polymerizes tubulin into microtubules in vitro, which induces mitotic arrest at the G2/M phase and results in inhibition of cell proliferation and cytotoxicity (1-3). Cell cycle arrest at nanomolar IC50 values have been observed in many cell types, including HeLa (IC50 = 32 nM), Hs578T (IC50 = 3 nM) (3), as well as the multiple myeloma cell lines U266 and RPMI 8226 (IC50 = ~1-10 nM) (4). Investigations have shown that both epothilone A and B competitively inhibit binding of taxol to microtubules in vitro(3). |
存放说明 | -20C |
纯度 | >99% |
计算分子量 | 507.68 g/mol |
参考文献 | 1 . Gerth, K. et al. (1996) J Antibiot (Tokyo) 49, 560-3. 2 . Goodin, S. et al. (2004) J Clin Oncol 22, 2015-25. 3 . Bollag, D.M. et al. (1995) Cancer Res 55, 2325-33. 4 . Lin, B. et al. (2005) Blood 105, 350-7. |
Chemical structure of epothilone B. | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Epothilone B (10 nM, 18 hr; +), using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 (upper) or Histone H3 (D1H2) XP® Rabbit mAb #4499 (lower). | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Epothilone B (10 nM, 18 hr; +), using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb #2914 (upper) or Aurora A (1F8) Mouse mAb #12100 and COX IV (3E11) Rabbit mAb #4850 (lower). |