| 货号 | 2222S |
| 供应商 | CST |
| 背景 | Anisomycin, an antibiotic produced by Streptomyces griseolus and S. roseochromogenes, was originally described to inhibit protein-protein synthesis at the translational level (1). More recently, it is has been well characterized to strongly activate the stress kinases SAPK/JNK and p38 MAPK, as well as p70/85 S6 kinase in mammalian cells, which results in the rapid induction of immediate-early (IE) genes, such as c-fos, fosB, c-jun, JunB, and JunD (1). Investigators have demonstrated that anisomycin acts as a potent signaling agonist, synergizing with growth factors and phorbol esters to superinduce these IE genes (1,2). Research studies have demonstrated that anisomycin induces apoptosis in many cancer cell lines (3-5). |
| 存放说明 | 4C |
| 参考文献 | 1 . Hazzalin, C.A. et al. (1998) Mol Cell Biol 18, 1844-54. 2 . Kardalinou, E. et al. (1994) Mol Cell Biol 14, 1066-74. 3 . Kochi, S.K. and Collier, R.J. (1993) Exp Cell Res 208, 296-302. 4 . Törocsik, B. and Szeberényi, J. (2000) Biochem Biophys Res Commun 278, 550-6. 5 . Curtin, J.F. and Cotter, T.G. (2002) Br J Cancer 87, 1188-94. |
Chemical structure of anisomycin.茴香霉素的化学结构。 | |
Western blot analysis of extracts from Jurkat cells, serum-starved overnight, pretreated with the indicated concentrations of SB202190 for 1 hr, and subsequently treated with Anisomycin (25 μg/ml, 30 min; +), using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 (upper) or p38 MAPK Antibody #9212 (lower).Western blot分析Jurkat细胞提取物，血清饥饿过夜，先用SB202190按照方案中设计好的浓度预处理1小时，再用茴香霉素(25 μg/ml, 30分钟; +)处理，使用的抗体是：Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511（上图）、p38 MAPK Antibody #9212（下图）。 |
