货号 | 13043S |
使用方法 | Vincristine is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 541.7 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1000 nM for 12-48 hr. |
供应商 | CST |
背景 | Vincristine is a vinca alkaloid isolated from the plant Catharanthus roseus that acts as a mitotic inhibitor blocking microtubule assembly in vitro (1,2). Research studies demonstrated that vincristine binds to and inhibits the addition of tubulin dimers to the assembly end of steady-state microtubules in a dose-dependent manner (Ki = ~85 nM) (1). Additional research suggests that high concentrations of vincristine depolymerize microtubules (2). Disruption of mitotic spindle function has been shown to cause mitotic arrest, inhibit cell proliferation, and induce apoptosis in numerous cell types (1-6). |
存放说明 | -20C |
纯度 | >99% |
计算分子量 | 923.04 g/mol |
参考文献 | 1 . Jordan, M.A. et al. (1985) Cancer Res 45, 2741-7. 2 . Jordan, M.A. et al. (1991) Cancer Res 51, 2212-22. 3 . Birk, M. et al. (2012) Int J Cancer 130, 798-807. 4 . Takano, Y. et al. (1993) Pathol Res Pract 189, 197-203. 5 . Tu, Y. et al. (2013) Int J Mol Med 31, 113-9. 6 . Groninger, E. et al. (2002) Int J Oncol 21, 1339-45. |
Chemical structure of vincristine. | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vincristine (10 nM, 18 hr; +), using Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb #3520 (upper) or NPM Antibody #3542 (lower). | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vincristine (10 nM, 18 hr; +), using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb #2914 (upper) or Aurora A (1F8) Mouse mAb #12100 and COX IV (3E11) Rabbit mAb #4850 (lower). |