| 货号 | 5934S |
| 供应商 | CST |
| 背景 | Okadaic acid is an inhibitor of the serine/theorine protein phosphatases PP1 (IC50 = 15-20 nM) and PP2A (IC50 = 0.1 nM), exhibiting much greater activity towards the latter. PP2A is completely inhibited at 1-2 nM, compared to greater than 1 µM for PP1 (1). This is unlike the protein phosphatase inhibitor Calyculin A, which potently inhibits both PP1 (IC50 = 2 nM) and PP2A (IC50 = 0.5-1 nM) (2). Okadaic acid does not effectively inhibit PP2C (3), acid and alkaline phosphatases, nor phosphotyrosine protein phosphatases (2,3). It has shown to inhibit PP2B, but at much higher concentrations than with PP1 and PP2A (3). Okadaic acid has also been described as a non-TPA type potent tumor promoter (4). These tumor-promoting properties and its ability to significantly increase protein phosphorylation make okadaic acid a very useful tool for studying cellular processes that are regulated by reversible phosphorylation of proteins (5). |
| 存放说明 | -20C |
| 参考文献 | 1 . Cohen, P. et al. (1989) FEBS Lett 250, 596-600. 2 . Ishihara, H. et al. (1989) Biochem Biophys Res Commun 159, 871-7. 3 . Bialojan, C. and Takai, A. (1988) Biochem J 256, 283-90. 4 . Suganuma, M. et al. (1988) Proc Natl Acad Sci U S A 85, 1768-71. 5 . Fernández, J.J. et al. (2002) Curr Med Chem 9, 229-62. |
Chemical structure of okadaic acid.冈田酸的化学结构。 | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Okadaic Acid (1 µM, 1 hr; +), using Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb #8654 (upper) or LATS1 (C66B5) Rabbit mAb #3477 (lower).Western blot分析HeLa细胞提取物，未处理(-)或用冈田酸(1 µM, 1小时; +)处理，使用的抗体是：Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb #8654（上图）、LATS1 (C66B5) Rabbit mAb #3477（下图）。 | |
Western blot analysis of extracts from HeLa cells, untreated or treated with Okadaic Acid (1 hr) at the indicated concentrations, using Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析HeLa细胞提取物，未处理或用冈田酸（1小时）按照方案设计好的浓度处理，使用的抗体是：Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381（上图）、β-Actin (D6A8) Rabbit mAb #8457（下图）。 |
