| 货号 | 9972S |
| 应用 | IF-IC |
| 供应商 | CST |
| 背景 | Brefeldin A (BFA) is a fungal metabolite demonstrated to reversibly interfere with anterograde transport from the endoplasmic reticulum to the Golgi apparatus (1,2). While initially isolated as an antibiotic (3), and does have a wide range of antibiotic activity, it is primarily used as a biological research tool for studying protein transport. Treatment leads to a rapid accumulation of proteins within the ER and collapse of the Golgi stacks. Treatment with BFA can also inhibit protein secretion (4) and prolonged exposure can induce apoptosis (5). The main target of BFA appears to be ADP-ribosylation factor (ARF), which is responsible for association of coat protein to the Golgi membrane (6,7).Brefeldin A (BFA)是一个真菌代谢物，其被证明可逆地干扰从内质网向高尔基体转运(1,2)。当起初分离的作为一个抗生素(3)，并且有一个广泛的抗生素活性，它主要作为研究蛋白转运的生物研究工具。BFA处理后导致在内质网上一个快速的蛋白堆积，并且高尔基体会被破坏。BFA的处理也能抑制蛋白分泌(4)和延长的给药时间能诱导凋亡(5)。BFA的主要目标出现是ADP-ribosylation factor (ARF)，该蛋白复制对外壳蛋白到高尔基膜的关联(6,7)。 |
| 存放说明 | -20C |
| 参考文献 | Klausner, R.D. et al. (1992) J Cell Biol 116, 1071-80. Pelham, H.R. (1991) Cell 67, 449-51. Tamura, G. et al. (1968) J Antibiot (Tokyo) 21, 160-1. Misumi, Y. et al. (1986) J Biol Chem 261, 11398-403. Shao, R.G. et al. (1996) Exp Cell Res 227, 190-6. Helms, J.B. and Rothman, J.E. (1992) Nature 360, 352-4. Randazzo, P.A. et al. (1993) J Biol Chem 268, 9555-63. |
Confocal immunofluorescent analysis of MCF-7 cells, untreated (left) or treated with Brefeldin A for 1.5 hours at 100 ng/ml (middle) or 20 μg/ml (right), using a Golgin-97 antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
