| 货号 | 2190S |
| 供应商 | CST |
| 背景 | Nocodazole is an anti-neoplastic agent that reversibly interferes with the polymerization of microtubules (1). Widely-used as a cell cycle synchronizing agent in cell biology labs to induce mitotic arrest, investigators have demonstrated that high concentrations of nocodazole induce microtubule depolymerization, whereas low concentrations alter spindle microtubule dynamics, but microtubules do not depolymerize (2-4). Recent research studies have demonstrated nocodazole to be a common inhibitor of various cancer-related kinases, including: ABL, c-KIT, BRAF, MEK1, MEK2, and MET (5). |
| 存放说明 | RT |
| 参考文献 | 1 . De Brabander, M.J. et al. (1976) Cancer Res 36, 905-16. 2 . Vasquez, R.J. et al. (1997) Mol Biol Cell 8, 973-85. 3 . Jordan, M.A. et al. (1992) J Cell Sci 102 ( Pt 3), 401-16. 4 . Blajeski, A.L. et al. (2002) J Clin Invest 110, 91-9. 5 . Park, H. et al. (2012) ChemMedChem 7, 53-6. |
Chemical structure of nocodazole.诺考达唑的化学结构。 | |
Western blot analysis of extracts from HeLa cells untreated (-) or treated with Nocodazole (0.1 μg/ml, 18 hr; +) or λ phosphatase (+), using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 (upper) or Histone H3 Antibody #9715 (lower).Western blot分析HeLa细胞提取物，未处理(-)或者用诺考达唑(0.1 μg/ml, 18小时; +)或λ磷酸酶(+)处理，使用的抗体是：Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377（上图）、Histone H3 Antibody #9715（下图）。 | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Nocodazole (0.1 μg/ml, 18 hr; +), using Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb #3520 (upper) or NPM Antibody #3542 (lower).Western blot分析HeLa细胞提取物，未处理(-)或用诺考达唑(0.1 μg/ml, 18小时; +)处理，使用的抗体是：Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb #3520（上图）、NPM Antibody #3542（下图）。 |
