| 货号 | 5083S |
| 供应商 | CST |
| 背景 | Erlotinib is a novel and potent ATP-competitive inhibitor of the EGFR kinase pathway. It inhibited EGFR autophosphorylation with an IC50 of 20 nM in vitro and inhibit purified EGFR kinase with an IC50 of 2 nM (1). Erlotonib is greater than 1000-fold more selective for EGFR than c-src and v-abl (1), ErbB-2, and ErbB-4 (2). Studies have shown that erlotinib inhibits growth and induces G1 cell cycle arrest in multiple cell types, many of which overexpress EGFR (1,3-5). |
| 存放说明 | -20C |
| 参考文献 | 1 . Moyer, J.D. et al. (1997) Cancer Res 57, 4838-48. 2 . Wood, E.R. et al. (2004) Cancer Res 64, 6652-9. 3 . Huether, A. et al. (2005) J Hepatol 43, 661-9. 4 . Ling, Y.H. et al. (2007) Mol Pharmacol 72, 248-58. 5 . Yamasaki, F. et al. (2007) Mol Cancer Ther 6, 2168-77. |
Western blot analysis of extracts from A-431 cells, serum-starved overnight and untreated or treated with hEGF #8916 (100 ng/ml, 5 min) either with or without Erlotinib pre-treatment (1 hr) at the indicated concentrations, using Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb #3777 (upper) or EGF Receptor (D38B1) XP® Rabbit mAb #4267 (lower).Western blot方法检测A-431细胞提取物，细胞经血清饥饿过夜后，不处理或用hEGF #8916 (100 ng/ml, 5 min)处理，用或不用指定浓度的厄洛替尼进行预处理(1 hr)，使用的抗体为Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb #3777 (上图)或EGF Receptor (D38B1) XP® Rabbit mAb #4267 (下图). | |
Chemical structure of Erlotinib.厄洛替尼的化学结构。 |
