| 货号 | 4174S |
| 描述 | TPA (12-O-tetradecanoylphorbol-13-acetate)/ PMA (phorbol-12-myristate-13-acetate) is soluble in DMSO and ethanol. DMSO solutions can be stored at -20C, in the absence of light, for at least six months. A 200 nM TPA treatment is standard to induce signaling. |
| 供应商 | CST |
| 背景 | TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is the most commonly used phorbol ester. It binds and activates protein kinase C, causing a wide range of effects in cells and tissues (1,2). TPA has been demonstrated to be a potent tumor promoter in mouse skin (3). Conversely, it has been shown to be an effective cancer therapeutic agent in myelocytic leukemia patients, and has been indicated as a potential colorectal cancer therapeutic (4-5). TPA has also been shown to increase white blood cell and neutrophil counts in solid tumor cancer patients (6). |
| 存放说明 | -20C |
| 参考文献 | Rebois, R.V. and Patel, J. (1985) J Biol Chem 260, 8026-31. Blumberg, P.M. (1980) Crit Rev Toxicol 8, 153-97. Fürstenberger, G. et al. (1981) Proc Natl Acad Sci U S A 78, 7722-6. Han, Z.T. et al. (1998) Proc Natl Acad Sci U S A 95, 5357-61. Lee, H.W. et al. (2002) J Biol Chem 277, 32624-31. Han, Z.T. et al. (1998) Proc Natl Acad Sci U S A 95, 5362-5. |
Western blot analysis of extracts from serum-starved 293 cells, untreated (lane 1), stimulated with TPA treatment (lanes 2 & 3), and treated with CIP and λ phosphatase (lane 3), using Phospho-PKC (pan) (βII Ser660) Antibody #9371 or PKCα Antibody #2056. | |
对血清饥饿的293细胞，未处理（列1），0.2uM TPA处理20分钟（列2,3），CIP/λ磷酸酶处理（列3），使用Phospho-PKC (pan) (βII Ser660)抗体#9371或PKCα抗体#2056进行Western blot分析。 |
