| 货号 | 9902S |
| 描述 | Calyculin A (Serine/Threonine Phosphatase Inhibitor) is a more potent phosphatase inhibitor than Okadaic acid (2). As shown by Western blot, treatment of cells with 100 nM Calyculin A for 30 minutes induces threonine phosphorylation, detected by Phospho-Threonine-Polyclonal Antibody #9381. IC50 values for inhibitory activity against PP1 are approximately 2 nM. IC50 values for inhibitory activity against PP2A are approximately 0.5 -1.0 nM.花萼海绵诱癌素A丝氨酸/苏氨酸抑制剂）与冈田软海绵酸相比较，是一种更加有潜力的磷酸酶抑制剂（2）。Western blot实验结果显示，用100nM花萼海绵诱癌素A处理细胞30分钟后，采用苏氨酸磷酸化多克隆抗体#9381可以检测到苏氨酸发生磷酸化。对抗PP1的抑制活性IC50值大约为2nM。对抗PP2A的抑制活性IC50值大约为0.5-1.0nM。 |
| 供应商 | CST |
| 背景 | Calyculin A inhibits the activity of protein phosphatases PP1 and PP2A (1,2). Unlike Okadaic acid, which reduces PP2A activity but has little effect on PP1 activity, Calyculin A inhibits both phosphatases (1). Neither Calyculin A nor Okadaic acid inhibit acid or alkaline phosphatases or phospho- tyrosine protein phosphatases (2).花萼海绵诱癌素A可以抑制PP1和PP2A蛋白磷酸酶的活性（1，2）。冈田软海绵酸仅降低PP2A的活性而对PP1的活性几乎没有影响，与其不同的是花萼海绵诱癌素A可以抑制这两种磷酸酶（1）。花萼海绵诱癌素A和冈田软海绵酸都不能抑制酸性或碱性磷酸酶或者酪氨酸蛋白磷酸酶的活性（2）。 |
| 存放说明 | -20C |
| 参考文献 | Resjö, S. et al. (1999) Biochem J 341 ( Pt 3), 839-45. Ishihara, H. et al. (1989) Biochem Biophys Res Commun 159, 871-7. |
Western blot analysis of extracts from A431 cells treated with varied concentrations of Okadaic Acid or calyculin A (30 minutes) using P-Thr-Polyclonal #9381.Wstern blot方法检测不同浓度冈田酸和花萼海绵体诱癌素A处理30分钟后的A431细胞提取物，使用的抗体为P-Thr-Polyclonal #9381。 | |
Western blot analysis of extracts from Jurkat cells, untreated or calyculin A-treated (0.1 µM for 45 minutes prior to lysis), using P-Thr-Polyclonal #9381. Proteins were separated by 2D electrophoresis prior to blotting. Western blot方法检测未处理和裂解前用0.1 µM 花萼海绵体诱癌素A处理45分钟的Jurkat细胞提取物，使用的抗体为P-Thr-Polyclonal #9381。蛋白样品先由2D电泳分离后再进行western blot分析。 |
