| 货号 | 54645S |
| 供应商 | CST |
| 背景 | Isolated from Streptomyces griseus, bafilomycin A1 is a macrolide antibiotic that reversibly inhibits late phase autophagy (1,2). By inhibiting H+-ATPase, bafilomycin A1 prevents re-acidification of synaptic vesicles following exocytosis and thereby blocks fusion of autophagosomes and lysosomes (2-5). Research studies demonstrate that bafilomycin A1 can also inhibit cell proliferation (4). |
| 存放说明 | -20C |
| 参考文献 | 1 . Werner, G. et al. (1984) J Antibiot (Tokyo) 37, 110-7. 2 . Yoshimori, T. et al. (1991) J Biol Chem 266, 17707-12. 3 . Bowman, E.J. et al. (1988) Proc Natl Acad Sci U S A 85, 7972-6. 4 . Ohkuma, S. et al. (1993) In Vitro Cell Dev Biol Anim 29A, 862-6. 5 . Yamamoto, A. et al. (1998) Cell Struct Funct 23, 33-42. |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Bafilomycin A1 (100 nM, 18 hr; right), using SQSTM1/p62 (D10E10) Rabbit mAb (IF Preferred) #7695 (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Bafilomycin A1 (200 nM, 18 hr; right), using NBR1 (D2E6) Rabbit mAb #9891 (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Chemical structure of Bafilomycin A1. |
