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货号： bs-2515R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2515R
- 英文名称
- CD2
- 中文名称
- CD2抗体
- 别 名
- T-cell surface antigen CD2; CD 2; CD2 antigen; CD2 molecule; LFA2; LFA3 receptor; Lymphocyte function antigen 2; p50; Rosette receptor; Sheep erythrocyte receptor; Sheep red blood cell receptor; SRBC; T cell surface antigen CD2; T cell surface antigen T11/Leu 5; T11; CD2_HUMAN.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 免疫学 干细胞 细胞表面分子 糖蛋白 自然杀伤细胞 淋巴细胞 t-淋巴细胞 b-淋巴细胞
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Horse,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 37kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CD2:281-351/351
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
CD2 is a surface antigen of the human T lymphocyte lineage that is expressed on all peripheral blood T cells. It is one of the earliest T cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes.
CD2 interacts with lymphocyte function associated antigen (LFA3) and CD48/BCM1 to mediate adhesion between T cells and other cell types. CD2 is implicated in the triggering of T cells, the cytoplasmic domain is implicated in the signaling function. It is useful for the identification of lymphomas and leukaemias of T cell origin.
Function:
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Subunit:
Interacts with CD2AP. Interacts with PSTPIP1.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Similarity:
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
SWISS:
P06729
Gene ID:
914
Database links:Entrez Gene: 914 Human
Entrez Gene: 12481 Mouse
Entrez Gene: 497761 Rat
Omim: 186990 Human
SwissProt: P06729 Human
SwissProt: P08920 Mouse
SwissProt: P08921 Rat
Unigene: 523500 Human
Unigene: 22842 Mouse
Unigene: 10328 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CD2分子又称T11、绵羊红细胞受体（ER）、淋巴细胞功能相关抗原2（LFA-2）和Leu5，是人T淋巴细胞表面的单链糖蛋白.CD2分子是分布于胸腺细胞及所有成熟T淋巴细胞和大部分自然杀伤细胞表面的特征性分化抗原。CD2具有调节T淋巴细胞分化、成熟、激活和凋亡等功能，CD2与LFA-3之间的粘附功能对于T淋巴细胞TCR识别外来抗原与APC细胞表面MHC抗原复合物、肿瘤抗原、病毒感染靶细胞以及同种异体抗原均有重要的辅助作用。
最近研究表明，CD48和CD59也是CD2的配体，参与T细胞的粘附和细胞间的相互作用,此外，CD2可以与其它膜分子如CD44、CD45的功能有关。CD2具有对T细胞的旁路激活途径作用。
- 产品图片
Blank control (Black line): Molt-4 (Black).
Primary Antibody (green line): Rabbit Anti-CD2 antibody (bs-2515R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.