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货号： bs-0498R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0498R
- 英文名称
- ADRB1
- 中文名称
- 肾上腺素能受体β1抗体
- 别 名
- beta 1 Adrenergic Receptor; beta 1 Adrenergic Receptor; ADRB1R; Adrenergic beta 1 receptor; B1AR; Beta 1 adrenoceptor; Beta 1 adrenoreceptor; Beta-1 adrenergic receptor; Beta-1 adrenoceptor; Beta-1 adrenoreceptor; BETA1AR; RHR; ADR B1; ADRB 1; ADRB1; ADRB1_HUMAN; ADRB1R; Adrenergic beta 1 receptor; Adrenoceptor beta 1.
- Specific References (2) | bs-0498R has been referenced in 2 publications.[IF=3.07] Deng, Guo-Hua, et al. "Exogenous norepinephrine attenuates the efficacy of sunitinib in a mouse cancer model." Journal of Experimental & Clinical Cancer Research 33:21 (2014). IHC-P ; Mouse.PubMed:24555849[IF=2.71] Bornholz, Beatrice, et al. "A standardised FACS assay based on native, receptor transfected cells for the clinical diagnosis and monitoring of β1-adrenergic receptor autoantibodies in human heart disease." Clinical Chemistry and Laboratory Medicine (CCLM). Human.PubMed:26408610
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 心血管 免疫学 神经生物学 信号转导 生长因子和激素 通道蛋白 细胞膜受体
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 52kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human ADRB1:181-250/477 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Beta-adrenergic receptors (Beta-1 adrenoreceptor) mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Subcellular location cell membrane; Multi-pass membrane protein. Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. Belongs to the G-protein coupled receptor 1 family.
Function:
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.
Subunit:
Interacts with GOPC, MAGI3 and DLG4.
Subcellular Location:
Cell membrane; Multi-pass membrane protein. Note=Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.
Post-translational modifications:
Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
Adrenergic receptor subfamily. ADRB1 sub-subfamily.
SWISS:
P08588
Gene ID:
153
Database links:Entrez Gene: 153 Human
Entrez Gene: 11554 Mouse
Entrez Gene: 24925 Rat
Omim: 109630 Human
SwissProt: P08588 Human
SwissProt: P34971 Mouse
SwissProt: P18090 Rat
Unigene: 99913 Human
Unigene: 46797 Mouse
Unigene: 87064 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
β1肾上腺素能受体（Beta-adrenergic Receptor-1 ）在接受交感神经节后纤维支配的各种器官中存在着与肾上腺素、去甲肾上腺素起反应的受体，称为肾上腺素能受体。其化学性质尚不清楚。用对药物反应的方法，肾上腺素能受体可分为α及β两个类型。肾上腺素对α、β及D型受体均起作用，而去甲肾上腺素主要对α型起作用。
β1AR所引起的反应为支气管扩张、血管扩张等，肾上腺素受体可被β13，4－dichloroisoproterenol（D－CI）等阻断药物所抑制。
- 产品图片
Sample:
Heart (Mouse) Lysate at 30 ug
Heart (Rat) Lysate at 30 ug
Primary: Anti- ADRB1(bs-0498R) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution
Predicted band size: 52 kD
Observed band size: 55 kD
Tissue/cell: skin of rat foot; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated(bs-0498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated(bs-0498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse kidney cell;
Incubation:
Red line: Anti-ADRB1 Polyclonal Antibody, FITC conjugated(bs-0498R-FITC) 1:200, 40 minutes at 37°C.
Green line: Isotype Control(bs-0295P-FITC, rabbit IgG/FITC) 1:200, 40 minutes at 37°C.