| 货号 | 14255S |
| 使用方法 | Vinblastine is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 550 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1000 nM for 12-48 hr. |
| 供应商 | CST |
| 背景 | Vinblastine is a vinca alkaloid derived from the plant Catharanthus roseus. Like other vinca alkaloids, it acts as a mitotic inhibitor blocking microtubule assembly in vitro (1,2). Investigators have demonstrated that vinblastine binds to and inhibits the addition of tubulin dimers to the assembly end of steady-state microtubules in a dose-dependent manner (Ki = ~178 nM) (1). This disruption of mitotic spindle causes mitotic arrest, inhibits cell proliferation, and induces apoptosis (1-3). Studies have suggested that high concentrations of vinblastine depolymerize microtubules, but this is not required for inhibition of cell proliferation (2). Treatment of cells with vinblastine has been shown to activate the SAPK/JNK pathway, leading to expression and phosphorylation of c-Jun (3,4) and phosphorylation of the anti-apoptotic proteins Bcl-2 and Bcl-xL (5,6). |
| 存放说明 | -20C |
| 纯度 | >98% |
| 计算分子量 | 909.05 g/mol |
| 参考文献 | 1 . Jordan, M.A. et al. (1985) Cancer Res 45, 2741-7. 2 . Jordan, M.A. et al. (1991) Cancer Res 51, 2212-22. 3 . Kolomeichuk, S.N. et al. (2008) FEBS J 275, 1889-99. 4 . Duan, L. et al. (2007) Biochem Pharmacol 73, 481-90. 5 . Fan, M. et al. (2000) J Biol Chem 275, 29980-5. 6 . Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8. |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vinblastine (10 nM, 18 hr; +), using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 (upper) or Histone H3 (D1H2) XP® Rabbit mAb #4499 (lower). | |
Western blot analysis of extracts from HeLa cells, untreated or treated with Vinblastine (18 hr) at the indicated concentrations, using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb #3270 (upper), c-Jun (60A8) Rabbit mAb, #9165 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
