| 货号 | 13630S |
| 使用方法 | IBMX is supplied as a lyophilized powder. For a 250 mM stock, reconstitute the 50 mg in 899.7 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1,000 µM for 15 min-4 hr. |
| 供应商 | CST |
| 背景 | 3-isobutyl-1-methylxanthine (IBMX) is a nonselective inhibitor of cAMP and cGMP phosphodiesterases (PDEs). Research studies show that IBMX inhibits many members of the PDE family, with the exceptions of PDE8A, PDE8B, and PDE9 (1-4). Treatment of cells with IBMX promotes accumulation of cAMP and cGMP, which leads to activation of cyclic-nucleotide-regulated protein kinases (5,6). Additional research indicates that IBMX can promote neuronal progenitor cell maturation in vitro(7). |
| 存放说明 | -20C |
| 纯度 | >98% |
| 计算分子量 | 222.30 g/mol |
| 参考文献 | 1 . Fawcett, L. et al. (2000) Proc Natl Acad Sci U S A 97, 3702-7. 2 . Fisher, D.A. et al. (1998) Biochem Biophys Res Commun 246, 570-7. 3 . Hayashi, M. et al. (1998) Biochem Biophys Res Commun 250, 751-6. 4 . Soderling, S.H. et al. (1998) J Biol Chem 273, 15553-8. 5 . Chanoit, G. et al. (2011) Cardiovasc Drugs Ther 25, 299-306. 6 . Zhang, R. et al. (2011) PLoS One 6, e20780. 7 . Lepski, G. et al. (2013) Front Cell Neurosci 7, 155. |
Chemical structure of IBMX. | |
Western blot analysis of extracts from C2C12 cells, untreated (-) or treated with Forskolin #3828 (10 μM, 30 min; +) with or without IBMX pretreatment (4 hr) at the indicated concentrations, using Phospho-VASP (Ser157) Antibody #3111 (upper), Phospho-VASP (Ser239) Antibody #3114 (middle), or VASP (9A2) Rabbit mAb #3132 (lower). |
