| 货号 | 5927S |
| 供应商 | CST |
| 背景 | Doxorubicin, an anthracycline antibiotic, inhibits DNA and RNA synthesis in mammalian cells and has been shown to be a very effective anti-tumor agent (1,2). Doxorubicin binds to nucleic acids by intercalating the DNA double helix and stabilizing topoisomerase II cleavage complexes, leading to DNA strand breaks at specific doxorubicin-induced sites (3). Doxorubin has been shown to inhibit DNA synthesis in a dose-dependent manner in MCF7 cells, which corresponds closely with growth inhibition (4). Researchers have also demonstrated that doxorubicin effectively inhibits human DNA topoisomerase I (5). |
| 存放说明 | -20C |
| 参考文献 | 1 . Kim, S.H. and Kim, J.H. (1972) Cancer Res 32, 323-5. 2 . Momparler, R.L. et al. (1976) Cancer Res 36, 2891-5. 3 . Capranico, G. et al. (1990) Nucleic Acids Res 18, 6611-9. 4 . Fornari, F.A. et al. (1994) Mol Pharmacol 45, 649-56. 5 . Foglesong, P.D. et al. (1992) Cancer Chemother Pharmacol 30, 123-5. |
Chemical structure of doxorubicin.多柔比星的化学结构。 | |
Western blot analysis of extracts from HeLa cells, serum-starved overnight and untreated or treated with Doxorubicin (5 μM) for the indicated times, using Phospho-p53 (Ser15) Antibody #9284, p53 Antibody #9282, Cleaved Caspase-3 (Asp175) Antibody #9661, or PARP Antibody #9542.Western blot分析HeLa细胞提取物，血清饥饿过夜，未处理或用多柔比星(5 μM)按照方案中设计好的时间处理，使用的抗体是：Phospho-p53 (Ser15) Antibody #9284、p53 Antibody #9282、Cleaved Caspase-3 (Asp175) Antibody #9661、PARP Antibody #9542。 | |
Western blot analysis of extracts from HeLa cells, untreated, treated with Doxorubicin (0.5 µM, 24 hr), or treated with Trichostatin A (TSA) #9950 (400 nM, 24 hr) and Doxorubicin (0.5 µM, 24 hr), using Acetyl-p53 (Lys382) Antibody #2525 (upper) or p53 (1C12) Mouse mAb #2524 (lower).Western blot分析HeLa细胞提取物，未处理，用多柔比星(0.5 µM, 24小时)处理或用Trichostatin A (TSA) #9950 (400 nM, 24小时)和多柔比星(0.5 µM, 24小时)处理，使用的抗体是：Acetyl-p53 (Lys382) Antibody #2525（上图）、p53 (1C12) Mouse mAb #2524（下图）。 |
