货号 | 19587-25mg |
描述 | Fluorescein-5-thiosemicarbazide (FTSC) is an amine-containing fluorescent probe. It can be reversibly coupled to aldehydes and ketones. FTSC has been used to fluorescently tag diverse molecules, including RNA, saccharides, sialylated glycoproteins, carbonylated proteins, and N-acetylneuraminic acid.1,2,3,4,5,6,7 |
别名 | FTSC; |
供应商 | Cayman |
应用文献 | |
1.Wu, T.-p.,Ruan, K.-c., and Liu, W.-y. A fluorescence-labeling method for sequencing small RNA on polyacrylamide gel. Nucleic Acids Research 24(17), 3472-3473 (1996). 2.Mohanty, J.G.,Bhamidipaty, S.,Evans, M.K., et al. A fluorimetric semi-microplate format assay of protein carbonyls in blood plasma. Analytical Biochemistry 400, 289-294 (2010). 3.Zhang, Y.,Wang, Z.,Zhang, X., et al. One-pot fluorescent labeling of saccharides with fluorescein-5-thiosemicarbazide for imaging polysaccharides transported in living cells. Carbohydr.Res. 346(14), 2156-2164 (2011). 4.Zhang, Y.,Yuan, J.,Song, J., et al. An efficient method for selectively imaging and quantifying in situ the expression of sialylated glycoproteins on living cells. Glycobiology 23(6), 643-653 (2013). 5.Havé, M.,Leitao, L.,Bagard, M., et al. Protein carbonylation during natural leaf senescence in winter wheat, as probed by fluorescein-5-thiosemicarbazide. Plant.Biol.(Stuttg) 17(5), 973-979 (2015). 6.Colombo, G.,Clerici, M.,Garavaglia, M.E., et al. A step-by-step protocol for assaying protein carbonylation in biological samples. J.Chromatogr.B.Analyt.Technol.Biomed.Life.Sci. 1019, (2016). 7.Dong, R.,Li, F.,Qin, S., et al. Dataset on inflammatory proteins expressions and sialic acid levels in apolipoprotein E-deficient mice with administration of N-acetylneuraminic acid and/or quercetin. Data Brief 8, (2016). | |
运输条件 | Wet ice in continental US; may vary elsewhere |
存放说明 | -20 |
纯度 | ≥95% |
计算分子量 | 457.9 |
分子式 | C21H15N3O5S • HCl |
稳定性 | ≥ 2 years |
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