| 货号 | 9900S |
| 供应商 | CST |
| 背景 | MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. |
| 存放说明 | -20C |
| 参考文献 | Crews, C.M. et al. (1992) Science 258, 478-80. Alessi, D.R. et al. (1994) EMBO J 13, 1610-9. Rosen, L.B. et al. (1994) Neuron 12, 1207-21. Cowley, S. et al. (1994) Cell 77, 841-52. |
Western blot analysis of extracts from COS cells transfected with pCMV-MKK3, untreated (-) or treated with Anisomycin #2222 (20 μg/ml, 30 min; +) and PD98059 pretreatment (indicated concentrations, 1 hr), using Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody #9231. | |
Western blot analysis of extracts from 293 cells, untreated (-) or NaCl-treated (0.7 M, 30 min; +) and PD98059 pretreatment (indicated concentrations, 1 hr), using Phospho-SEK1/MKK4 (Thr261) Antibody #9151. |
