| 货号 | 2194S |
| 供应商 | CST |
| 背景 | MG-132 is a potent proteasome and calpain inhibitor. Research studies have shown that MG-132 prevents degradation of the proteasome substrates SucLLVY-MCA and ZLLL-MCA with an IC50 of 850 nM and 100 nM, respectively, as well as inhibits casein-degrading activity of m-calpain with an IC50 of 1.25 µM (1). Investigators have demonstrated that MG-132 inhibits TNF-α-induced NF-κB activation and IL-8 release in A549 cells (2). Proteasome inhibition with MG-132 prevents degradation of short-lived proteins, which correlates with increased expression of HSP and ER chaperone proteins (3). MG-132 induces apoptosis in Hep G2 cells in a time- and dose-dependent manner (4). Proteasome inhibitors like MG-132 are important research tools for studying cellular degradation of the ubiquitin-proteasome pathway. |
| 存放说明 | -20C |
| 参考文献 | 1 . Tsubuki, S. et al. (1996) J Biochem 119, 572-6. 2 . Fiedler, M.A. et al. (1998) Am J Respir Cell Mol Biol 19, 259-68. 3 . Bush, K.T. et al. (1997) J Biol Chem 272, 9086-92. 4 . Emanuele, S. et al. (2002) Int J Oncol 21, 857-65. |
Chemical structure of MG-132.MG-132的化学结构。 | |
Western blot analysis of extracts from HT-1080 cells, untreated (-) or treated with MG-132 (10 μM, 4 hr; +), using Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb #3300.Western blot分析HT-1080细胞的提取物，未处理(-)或用MG-132 (10 μM, 4小时; +)处理，使用的抗体是：Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb #3300。 | |
Western blot analysis of extracts from 293 and HeLa cells, untreated (-) or treated with MG-132 (50 µM, 90 min; +), using Ubiquitin (P4D1) Mouse mAb #3936.Western blot分析293和HeLa细胞的提取物，未处理(-)或用MG-132 (50 µM, 90分钟; +)处理，使用的抗体是：Ubiquitin (P4D1) Mouse mAb #3936。 |
