| 货号 | 16123-500ug |
| 描述 | Coelenterazine is a luciferin, a light-emitting biomolecule that serves as a substrate for luciferases or as a constituent of photoproteins, including aequorin.1 The oxidation of coelenterazine to coelenteramide is accompanied by emission of blue light (emission maximum, 460-470 nm). Luciferase-mediated oxidation of coelenterazine or a derivative is used as an energy donor, typically to a form of green or yellow fluorescent protein, in bioluminescent resonance energy transfer studies.2,3 Alternatively, the calcium-mediated release of coelenterazine from aequorin, followed by non-enzymatic oxidation of this compound, results in bioluminescence. As light emission depends on both calcium and cellular redox status, this reaction is used to non-fluorescently detect changes in calcium level and redox status.4,5 |
| 别名 | Luciferin (Oplophorus);Preluciferin (Watasenia); |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010). 2.Pfleger, K.D.G. and Eidne, K.A. Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET). Nature Methods 3(3), 165-174 (2006). 3.Dacres, H.,Dumancic, M.M.,Horne, I., et al. Direct comparison of bioluminescence-based resonance energy transfer methods for monitoring of proteolytic cleavage. Analytical Biochemistry 385, 194-202 (2009). 4.Stables, J.,Green, A.,Marshall, F., et al. A bioluminescent assay for agonist activity at potentially any G-protein-coupled receptor. Analytical Biochemistry 252, 115-126 (1997). 5.Teranishi, K. and Shimomura, O. Coelenterazine analogs as chemiluminescent probe for superoxide anion. Analytical Biochemistry 249, 37-43 (1997). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥85% |
| 计算分子量 | 423.5 |
| 分子式 | C26H21N3O3 |
| CAS号 | 55779-48-1 |
| 稳定性 | ≥ 2 years |
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