| 货号 | 9885S |
| 供应商 | CST |
| 背景 | Roscovitine is a cell permeable reversible selective inhibitor of cyclin-dependent kinases CDK1 (cdc2), CDK2 and CDK5 (1). A purine analog, this drug competes for the binding site of ATP in the catalytic cleft. Treatment of cultured cells with roscovitine can cause cell cycle arrest or apoptosis (1-4). The IC50 for cdc2 activity is 0.65 μM in vitro (1).核抑制剂是一种能透过细胞的周期蛋白依赖性激酶CDK1 (cdc2)，CDK2和CDK5的选择性抑制剂(1)。作为一种嘌呤类似物，该药物竞争性结合催化裂口的ATP位点。使用roscovitine处理培养的细胞能够引起细胞周期捕获或者凋亡(1-4)。该药对于cdc2活性的体外IC50是0.65 μM (1)。 |
| 存放说明 | -20C |
| 参考文献 | Meijer, L. et al. (1997) Eur J Biochem 243, 527-36. Whittaker, S.R. et al. (2007) Cell Cycle 6, 3114-31. Dey, A. et al. (2008) Cell Death Differ 15, 263-73. Wojciechowski, J. et al. (2003) Int J Cancer 106, 486-95. |
Western blot analysis of extracts from HeLa cells treated with Roscovitine (20 μM) for the indicated times using Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb #2325 (left) or Phospho-(Ser) CDKs Substrate Antibody #2324 (right).Western blot 方法检测Roscovitine (20 μM)处理不同时间的Hela细胞提取物，使用的抗体为Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb #2325 (左图) 或 Phospho-(Ser) CDKs Substrate Antibody #2324 (右图). | |
Structure of Roscovitine。Roscovitine的结构。 | |
Western blot analysis of extracts from HeLa cells treated with Roscovitine (20 μM) for the indicated times using an antibody to Rb.Western blot 方法检测 Roscovitine (20 μM)处理不同时间的Hela细胞提取物，检测Rb蛋白。 |
