DAF-2 diacetate
货号：
85165-100ug 基本售价：
588.0 元 规格：
100 ug
产品信息
概述| 货号 | 85165-100ug |
| 描述 | DAF-2 diacetate is a sensitive fluorescent indicator for the detection and bioimaging of nitric oxide (NO).1,2 It is a cell-permeable derivative of DAF-2. Upon entry into the cell, DAF-2 diacetate is transformed into the less cell-permeable DAF-2 by cellular esterases thus preventing loss of signal due to diffusion of the molecule from the cell. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nm, respectively.2 At neutral pH the detection limit for NO is 2-5 nM. DAF-2 diacetate can be utilized in cells which produce small amounts of NO, such as endothelial cells, as well as in cells which generate large amount of NO, such as macrophages.1,2 |
| 别名 | 4,5-Diaminofluorescein diacetate; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Kojima, H.,Sakurai, K.,Kikuchi, K., et al. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore. Chemical & Pharmaceutical Bulletin 46, 373-375 (1998).
2.Nakatsubo, N.,Kojima, H.,Kikuchi, K., et al. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: Diaminofluoresceins. FEBS Letters 427, 263-266 (1998).
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| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥95% |
| 计算分子量 | 446.4 |
| 分子式 | C24H18N2O7 |
| CAS号 | 205391-02-2 |
| 稳定性 | ≥ 1 year |
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