| 货号 | 14459-1mg |
| 描述 | During apoptosis, activated caspase-3 cleaves several substrates, including poly(ADP-ribose) polymerase, which it specifically targets at the amino sequence DEVD.1 Ac-DEVD-AFC is a fluorogenic substrate for activated caspase-3 (Km = 9.7 µM), as well as related caspases.2,3,4 Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm. |
| 别名 | N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Trifluoromethylcoumarin;Caspase-3 Substrate (Fluorogenic); |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Lazebnik, Y.A.,Kaufmann, S.H.,Desnoyers, S., et al. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371, 346-347 (1994). 2.Xiang, J.,Chao, D.T. and Korsmeyer, S.J. BAX-induced cell death may not require interleukin 1β-converting enzyme-like proteases. Proceedings of the National Academy of Sciences of the United States of America 93, 14559-14563 (1996). 3.Chandler, J.M.,Cohen, G.M. and MacFarlane, M. Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver. The Journal of Biological Chemisty 273(18), 10815-10818 (1998). 4.Marcelli, M.,Cunningham, G.R.,Haidacher, S.J., et al. Caspase-7 is activated during lovastatin-induced apoptosis of the prostate cancer cell line LNCaP. Cancer Research 58(1), 76-83 (1998). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 729.6 |
| 分子式 | C30H34F3N5O13 |
| CAS号 | 201608-14-2 |
| 稳定性 | ≥ 2 years |
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