| 货号 | 16529-25mg |
| 描述 | Methanethiosulfonates (MTS) are sulfhydryl-reactive compounds that form mixed disulfide linkages and are commonly used to study cysteine residues on proteins. Sodium (2-sulfonatoethyl)methanethiosulfonate (MTSES) is a negatively-charged, membrane impermeant MTS. It is highly reactive with ionized thiolates but not with unionized thiols and, therefore, targets sulfhydryl groups accessible from the aqueous medium. MTSES is used to probe the structural and functional properties of native proteins, particularly those associated with membranes, including channels and transporters.1,2,3 In addition, charged MTS compounds like MTSES are combined with cysteine scanning mutagenesis to study non-cysteine residues.4,5 |
| 别名 | Sodium (2-Sulfonatoethyl)methanethiosulfonate; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Lang, R.J.,Harvey, J.R. and Mulholland, E.L. Sodium (2-sulfonatoethyl) methanethiosulfonate prevents S-nitroso-L-cysteine activation of Ca2+-activated K+ (BKCa) channels in myocytes of the guinea-pig taenia caeca. British Journal of Pharmacology 139(6), 1153-1163 (2003). 2.Li, R.A.,Tsushima, R.G.,Kallen, R.G., et al. Pore residues critical for μ-CTX binding to rat skeletal muscle Na+ channels revealed by cysteine mutagenesis. Biophysical Journal 73(4), 1874-1884 (1997). 3.Guan, L. and Kaback, H.R. Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins. Nature Protocols 2(8), 2012-2017 (2007). 4.Engh, A.M. and Maduke, M. Cysteine accessibility in ClC-0 supports conservation of the ClC intracellular vestibule. Journal of General Physiology 125(6), 601-617 (2014). 5.Liu, X.,Alexander, C.,Serrano, J., et al. Variable reactivity of an engineered cysteine at position 338 in cystic fibrosis transmembrane conductance regulator reflects different chemical states of the thiol. The Journal of Biological Chemisty 281(12), 8275-8285 (2006). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 242.3 |
| 分子式 | C3H7O5S3 • Na |
| CAS号 | 184644-83-5 |
| 稳定性 | ≥ 2 years |
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