| 货号 | 16527-10mg |
| 描述 | Carbohydrates conjugated with 2-chloro-4-nitrophenyl (CNP) serve as chromogenic substrates in assays for enzymes that release CNP from the conjugated carbohydrate.1,2 For example, CNP-β-D-maltoheptaoside and CNP-α-maltotrioside are chromogenic substrates for α-amylase.3,4 2-Chloro-4-nitrophenyl-α-D-glucopyranoside is a conjugate of CNP and α-D-glucose. The related conjugate between CNP and β-D-glucose, 2-chloro-4-nitrophenyl β-D-glucopyranoside, is used as a chromogenic substrate for glycosyltransferases.5,6 This product is a chromogenic substrate for enzymes that target the linkage between CNP and α-D-glucose. |
| 别名 | CNP-α-D-glucopyranoside; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Makise, J.,Saito, E.,Obuchi, M., et al. Kinetic rate assay of urinary N-acetyl-β-D-glucosaminidase with 2-chloro-4-nitrophenyl-N-acetyl-β-D-glucosaminide as substrate. Clinical Chemistry 34(10), 2140-2143 (1988). 2.Matsui, I. and Svensson, B. Improved activity and modulated action pattern obtained by random mutagenesis at the fourth β-α loop involved in substrate binding to the catalytic (β/α)8-barrel domain of barley α-amylase 1. The Journal of Biological Chemisty 272(36), 22456-22463 (1997). 3.Mori, H.,Bak-Jensen, K.S.,Gottschalk, T.E., et al. Modulation of activity and substrate binding modes by mutation of single and double subsites +1/+2 and -5/-6 of barley α-amylase 1. European Journal of Biochemistry 268(24), 6545-6558 (2001). 4.Al-Dabbas, M.M.,Kitahara, K.,Suganuma, T., et al. Antioxidant and alpha-amylase inhibitory compounds from aerial parts of Varthemia iphionoides Boiss. Bioscience, Biotechnology, and Biochemistry 70(9), 2178-2184 (2006). 5.Gantt, R.W.,Peltier-Pain, P.,Singh, S., et al. Broadening the scope of glycosyltransferase-catalyzed sugar nucleotide synthesis. Proceedings of the National Academy of Sciences of the United States of America 110(19), 7648-7653 (2013). 6.Gantt, R.W.,Peltier-Pain, P.,Cournoyer, W.J., et al. Using simple donors to drive the equilibria of glycosyltransferase-catalyzed reactions. Nature Chemical Biology 7(10), 685-691 (2011). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 335.7 |
| 分子式 | C12H14ClNO8 |
| CAS号 | 119047-14-2 |
| 稳定性 | ≥ 2 years |
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