| 货号 | 15551-5mg |
| 描述 | BAPTA (Item No. 11706) is membrane-impermeable calcium chelator that binds extracellular calcium ions (Kd = 0.11 μM), with selectivity over magnesium ions or protons.1,2 BAPTA and its derivatives can also be used as calcium indicators, since the absorption maximum for BAPTA changes when it is complexed with calcium (absorption maxima free/complexed = 254/274 nm, emission maxima free/complexed = 363/363 nm).3 BAPTA AM is a cell permeable analog of BAPTA that binds calcium only after the acetoxymethyl group is removed by cytoplasmic esterases.4 It is commonly used at 10-100 μM to evaluate the role of intracellular calcium in cell signaling.5,4,6,7 It can also be used in animals.4 BAPTA AM also inhibits voltage-gated potassium (Kv) channels, including Kv1.3, Kv1.5, and Kv11.1 (Ki = 1.45, 1.23, and 1.30 μM, respectively).8 |
| 别名 | BAPTA Acetoxymethyl ester; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010). 2.Grynkiewicz, G.,Poenie, M. and Tsien, R.Y. A new generation of Ca2+ indicators with greatly improved fluorescence properties. The Journal of Biological Chemisty 260(6), 3440-3450 (1985). 3.Tsien, R.Y. Intracellular measurements of ion activities. Annu.Rev.Biophys.Bioeng. 12, 91-116 (1983). 4.Tymianski, M.,Spigelman, I.,Zhang, L., et al. Mechanism of action and persistence of neuroprotection by cell-permeant Ca2+ chelators. Journal of Cerebral Blood Flow and Metabolism 14(6), 911-923 (1994). 5.Verrière, V.,Higgins, G.,Al-Alawi, M., et al. Lipoxin A4 stimulates calcium-activated chloride currents and increases airway surface liquid height in normal and cystic fibrosis airway epithelia. PLoS One 7(5), e37746 (2012). 6.Chang, M.G.,Chang, C.Y.,de Lange, E., et al. Dynamics of early afterdepolarization-mediated triggered activity in cardiac monolayers. Biophysical Journal 102(12), 2706-2714 (2012). 7.Bray, G.E.,Ying, Z.,Baillie, L.D., et al. Extracellular pH and neuronal depolarization serve as dynamic switches to rapidly mobilize trkA to the membrane of adult sensory neurons. Journal of Neuroscience 33(19), 8202-8215 (2013). 8.Tang, Q.,Jin, M.W.,Xiang, J.Z., et al. The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells. Biochemical Pharmacology 74(11), 1596-1607 (2007). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 764.7 |
| 分子式 | C34H40N2O18 |
| CAS号 | 126150-97-8 |
| 稳定性 | ≥ 2 years |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |