| 货号 | 14320-25g |
| 描述 | Brilliant Blue G is a dye which is commonly used in laboratories to stain or quantify proteins. A variety of methods have been developed for using Brilliant Blue G to stain gels with ease and sensitivity.1,2,3,4 The Bradford assay is a standard, rapid dye-binding assay that uses Brilliant Blue G to quantify the amount of protein in a solution.5 This compound also acts as a selective inhibitor of the P2X purinoceptor channel P2X7(IC50s = 10.1 and 265 nM for rat and human P2X7, respectively).6 In mice, it inhibits interleukin-1β expression and reduces neurological injury secondary to traumatic brain injury.7 |
| 别名 | Acid Blue 90;CBBG;Coomassie Brilliant Blue G-250;NSC 328382; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Diezel, W.,Kopperschläger, G. and Hofmann, E. An improved procedure for protein staining in polyacrylamide gels with a new type of Coomassie brilliant blue. Analytical Biochemistry 48, 617-620 (1972). 2.Neuhoff, V.,Stamm, R. and Eibl, H. Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: A systematic analysis. Electrophoresis 6, 427-448 (1985). 3.Candiano, G.,Bruschi, M.,Musante, L., et al. Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25, 1327-1333 (2004). 4.Pink, M.,Verma, N.,Rettenmeier, A.W., et al. CBB staining protocol with higher sensitivity and mass spectrometric compatibility. Electrophoresis 31, 593-598 (2010). 5.Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254 (1976). 6.Jiang, L.H.,Mackenzie, A.B.,North, R.A., et al. Brilliant Blue G selectively blocks ATP-gated rat P2X7 receptors. Molecular Pharmacology 58(1), 82-88 (2000). 7.Kimbler, D.E.,Shields, J.,Yanasak, N., et al. Activation of P2X7 promotes cerebral edema and neurological injury after traumatic brain injury in mice. PLoS One 7(7), (2012). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | 22 |
| 计算分子量 | 854 |
| 分子式 | C47H48N3O7S2 • Na |
| CAS号 | 6104-58-1 |
| 稳定性 | ≥ 2 years |
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