| 货号 | 11861-5mg |
| 描述 | Brefeldin A (BFA) is a natural fungal metabolite which has been used extensively to study intracellular transport by vesicles or endosomes. Early studies demonstrated that BFA reversibly interferes with protein trafficking and secretion mediated by the Golgi apparatus and endoplasmic reticulum.1,2,3 BFA directly and reversibly inhibits Sec7 domain-containing guanine-exchange factors which are necessary for ADP-ribosylation factor activation associated with vesicular transport (IC50 = ~10 μM).4,5,6 BFA is used to study endosomal trafficking and function in cells of plants as well as those of fungi, invertebrates, and vertebrates.7,8 |
| 别名 | Ascotoxin;BFA;Cyanein;Decumbin;Nectrolide;NSC 56310;NSC 89671;NSC 107456;NSC 244390;Synergisidin; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Fujiwara, T.,Oda, K.,Yokota, S., et al. Brefeldin A causes disassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum. The Journal of Biological Chemisty 263(34), 18545-18552 (1988). 2.Ayala, J. Transport and internal organization of membranes: Vesicles, membrane networks and GTP-binding proteins. Journal of Cell Science 107, 753-763 (1994). 3.Thyberg, J., and Moskalewski, S. Role of microtubules in the organization of the Golgi complex. Experimental Cell Research 246, 263-279 (1999). 4.Klausner, R.D.,Donaldson, J.G., and Lippincott-Schwartz, J. Brefeldin A: Insights into the control of membrane traffic and organelle structure. Journal of Cell Biology 116(5), 1071-1080 (1992). 5.Moss, J., and Vaughan, M. Activation of toxin ADP-ribosyltransferases by eukaryotic ADP-ribosylation factors. Molecular and Cellular Biochemistry 193, 153-157 (1999). 6.Vigil, D.,Cherfils, J.,Rossman, K.L., et al. Ras superfamily GEFs and GAPs: Validated and tractable targets for cancer therapy? Nature Reviews.Cancer 10(12), 842-857 (2010). 7.Robinson, D.G.,Jiang, L., and Schumacher, K. The endosomal system of plants: Charting new and familiar territories. Plant Physiology 147, 1482-1492 (2008). 8.Nebenführ, A.,Ritzenthaler, C., and Robinson, D.G. Brefeldin A: Deciphering and enigmatic inhibitor of secretion. Plant Physiology 130, 1102-1108 (2002). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 280.4 |
| 分子式 | C16H24O4 |
| CAS号 | 20350-15-6 |
| 稳定性 | ≥ 2 years |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |