| 货号 | 16383-5mg |
| 描述 | Fluorescein-5-maleimide is an activated fluorescent molecule (excitation: 494 nm, emission: 519 nm) that is used to easily conjugate fluorescein to proteins.1,2,3 The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond.4 The compound can be used to label proteins for the detection of conformational changes, assembly of multisubunit complexes, and ligand-binding processes.5 |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Curtis, S.K. and Cowden, R.R. Demonstration of sulfhydryl and disulfide groups by a fluorescent maleimide procedure. Histochemistry 68(1), 23-28 (1980). 2.Elliott, J.T.,Tona, A. and Plant, A.L. Comparison of reagents for shape analysis of fixed cells by automated fluorescence microscopy. Cytometry 52(2), 90-100 (2003). 3.Lepedda, A.j.,Zinellu, A.,Nieddu, G., et al. Protein sulfhydryl group oxidation and mixed-disulfide modifications in stable and unstable human carotid plaques. Oxid.Med.Cell.Longev. 2013, 1-8 (2013). 4.Bigelow, D.J. and Inesi, G. Frequency-domain fluorescence spectroscopy resolves the location of maleimide-directed spectroscopic probes within the tertiary structure of the Ca-ATPase of sarcoplasmic reticulum. Biochemistry 30(8), 2113-2125 (1991). 5.Taraska, J.W. and Zagotta, W.N. Fluorescence applications in molecular neurobiology. Neuron 66(2), 170-189 (2010). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥90% |
| 计算分子量 | 427.4 |
| 分子式 | C24H13NO7 |
| CAS号 | 75350-46-8 |
| 稳定性 | ≥ 2 years |
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