| 货号 | 85100-1mg |
| 描述 | DHR is a cell-permeable fluorogenic probe that is used as an indicator of intracellular peroxynitrite formation.1 It is oxidized by peroxynitrite to the highly fluorescent product rhodamine in vitro. Neither nitric oxide, superoxide, nor hydrogen peroxide alone appear to oxidize DHR.1 Formation of rhodamine can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 78,800 M−1cm−1).2,3,1 DHR has been used to investigate reactive oxygen intermediates produced by endothelial cells, eosinophils, and reactive microglia.4,5,6 |
| 别名 | DHR 123; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: Implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide: Biology and Chemistry 1, 145-157 (1997). 2.Briviba, K.,Roussyn, I.,Sharov, V.S., et al. Attenuation of oxidation and nitration reactions of peroxynitrite by selenomethionine, selenocystine and ebselen. Biochemistry Journal 319, 13-15 (1996). 3.Sies, H.,Sharov, V.S.,Klotz, L.O., et al. Glutathione peroxidase protects against peroxynitrite-mediated oxidations. A new function for selenoproteins as peroxynitrite reductase. The Journal of Biological Chemisty 272, 27812-27817 (1997). 4.Handa, O.,Stephen, J. and Cepinskas, G. Role of endothelial nitric oxide synthase-derived nitric oxide in activation and dysfunction of cerebrovascular endothelial cells during early onsets of sepsis. American Journal of Physiology.Heart and Circulatory Physiology 295, H1712-1719 (2008). 5.Lacy, P.,Latif, D.A.,Steward, M., et al. Divergence of mechanisms regulating respiratory burst in blood and sputum eosinophils and neutophils from atopic subjects. Journal of Immunology 170, 2670-2679 (2003). 6.Li, J.,Baud, O.,Volpe, J.J., et al. Peroxynitrite generated by inducible nitric oxide synthase and NADPH oxidase mediates microglial toxicity to oligodendrocytes. Proceedings of the National Academy of Sciences of the United States of America 102(28), 9936-9941 (2005). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 346.4 |
| 分子式 | C21H18N2O3 |
| CAS号 | 109244-58-8 |
| 稳定性 | ≥ 2 years |
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