TMB (hydrochloride hydrate)
货号:
70450-100mg 基本售价:
406.0 元 规格:
100 mg
产品信息
概述货号 | 70450-100mg |
描述 | TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000).1 The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB.1,2 Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase. |
性能供应商 | Cayman |
应用文献 |
1.Josephy, P.D.,Eling, T. and Mason, R.P. The horseradish peroxidase-catalyzed oxidation of 3,5,3,5-tetramethylbenzidine. Free radical and charge-transfer complex intermediates. The Journal of Biological Chemisty 257, 3669-3675 (1982). 2.Marquez, L.A. and Dunford, H.B. Mechanism of the oxidation of 3,5,3,5-tetramethylbenzidine by myeloperxidase determined by transient-and steady-state kinetics. Biochemistry 36, 9349-9355 (1997).
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运输条件 | Room temperature in continental US; may vary elsewhere |
存放说明 | -20 |
纯度 | ≥98% |
计算分子量 | 349.3 |
分子式 | C16H20N2 • 2HCl [2H2O] |
CAS号 | 207738-08-7 |
稳定性 | ≥ 1 year |
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