| 货号 | 18140-1mg |
| 描述 | 5-Bromouridine 5-triphosphate (5-BrUTP) is a brominated form of UTP that is used to label RNA during transcription. 5-BrUTP in newly-transcribed RNA is then evaluated immunologically with antibodies. This approach has been used to detect or measure RNA transcription with a variety of imaging and molecular methods.1,2,3,4,5 |
| 别名 | 5-BrUTP; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Abe, K.i.,Inoue, A.,Suzuki, M.G., et al. Global gene silencing is caused by the dissociation of RNA polymerase II from DNA in mouse oocytes. Journal of Reproduction and Development 56(5), 502-507 (2010). 2.Eskiw, C.H.,Rapp, A.,Carter, D.R.F., et al. RNA polymerase II activity is located on the surface of protein-rich transcription factories. Journal of Cell Science 121, 1999-2007 (2008). 3.Heinrich, B.S.,Cureton, D.K.,Rahmeh, A.A., et al. Protein expression redirects vesicular stomatitis virus RNA synthesis to cytoplasmic inclusions. PLoS Pathogens 6(6), 1-14 (2010). 4.Javed, A.,Zaidi, S.K.,Gutierrez, S.E., et al. In situ immunofluorescence analysis: Analyzing RNA synthesis by 5-bromouridine-5-triphosphate labeling. Methods in Molecular Biology 285, 29-31 (2004). 5.Thiry, M.,Cheutin, T.,ODonohue, M.F., et al. Dynamics and three-dimensional localization of ribosomal RNA within the nucleolus. RNA 6, 1750-1761 (2000). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥95% |
| 计算分子量 | 629 |
| 分子式 | C9H11BrN2O15P3 • 3Na |
| 稳定性 | ≥ 2 years |
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