Everolimus【科瑞奥博】

产品中心

当前位置：首页>产品中心

Everolimus

货号： 12017S 基本售价： 378.0 元规格： 1mg

产品信息

概述
货号	12017S

性能
供应商	CST
背景	Everolimus, also known as RAD001, is an immunosuppressant analog of rapamycin. Everolimus forms a complex with FKBP12 with an IC50 = ~2 nM in FK506 competitive binding assays (1,2), and this complex then binds to and inhibits mTORC1 (3). Studies have shown that everolimus treatment of cells can lead to the dephosphorylation of mTOR downstream targets (4-7), inhibition of VEGF- and bFRF-stimulated proliferation in HUVE cells (6), and reduction of hypoxia-induced HIF-1 protein levels (7).
存放说明	-20C
参考文献	1 . Sedrani, R. et al. (1998) Transplant Proc 30, 2192-4. 2 . Schuler, W. et al. (1997) Transplantation 64, 36-42. 3 . Houghton, P.J. (2010) Clin Cancer Res 16, 1368-72. 4 . Rosich, L. et al. (2012) Clin Cancer Res 18, 5278-89. 5 . Cao, C. et al. (2006) Cancer Res 66, 10040-7. 6 . Lane, H.A. et al. (2009) Clin Cancer Res 15, 1612-22. 7 . Knaup, K.X. et al. (2009) Mol Cancer Res 7, 88-98.

参考图片

Chemical structure of everolimus.

everolimus的化学结构。

Western blot analysis of extracts from HeLa cells, serum-starved overnight and treated with hIGF-I #8917 (100 ng/ml, 10 min) either with or without Everolimus pretreatment (1 hr) at the indicated concentrations, using Phospho-p70 S6 Kinase (Thr389) Antibody #9205 (upper) or p70 S6 Kinase (49D7) Rabbit mAb #2708 (lower).

Western blot方法分别检测血清饥饿过夜以及hIGF-I #8917 (100 ng/ml, 10 min) 处理的293细胞提取物，上述细胞包含经过和未经所示浓度的Everolimus预处理两种情况，使用的抗体为Phospho-p70 S6 Kinase (Thr389) Antibody #9205 (上图)或p70 S6 Kinase (49D7) Rabbit mAb兔单抗 #2708 (下图)。

Western blot analysis of extracts from HeLa cells, serum-starved overnight and treated with hIGF-I #8917 (100 ng/ml, 10 min) either with or without Everolimus pretreatment (1 hr) at the indicated concentrations, using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).

Western blot方法分别检测血清饥饿过夜以及hIGF-I #8917 (100 ng/ml, 10 min) 处理的HeLa细胞提取物，上述细胞包含经过和未经所示浓度的Everolimus预处理两种情况，使用的抗体为Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗 #4858 (上图)或S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗 #2217 (下图)。