2,7-Dichlorodihydrofluorescein diacetate
货号：
85155-50mg 基本售价：
364.0 元 规格：
50 mg
产品信息
概述| 货号 | 85155-50mg |
| 描述 | DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation.1,2 DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ɛ = 59,500 M−1cm−1).1,2 |
| 别名 | DCFH;DCFH-DA; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: Implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide: Biology and Chemistry 1, 145-157 (1997).
2.Kooy, N.W.,Royall, J.A., and Ischiropoulos, H. Oxidation of 2,7-dichlorofluorescin by peroxynitrite. Free Radical Research 27, 245-254 (1997).
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| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥95% |
| 计算分子量 | 487.3 |
| 分子式 | C24H16Cl2O7 |
| CAS号 | 4091-99-0 |
| 稳定性 | ≥ 1 year |
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