Puromycin (hydrochloride)
货号：
13884-25mg 基本售价：
364.0 元 规格：
25 mg
产品信息
概述| 货号 | 13884-25mg |
| 描述 | Puromycin is an aminonucleoside antibiotic, derived from S. alboniger, that blocks protein synthesis in both prokaryotes and eukaryotes by causing premature termination of nascent polypeptide chains.1 A portion of the puromycin molecule resembles the 3 end of aminoacylated tRNA.2 It enters the A site of the ribosome and attaches to the growing polypeptide chain at the peptidyl-transferase center, causing the formation of a puromycylated nascent chain and premature chain release.3 The 3 position contains an amide linkage instead of the normal ester linkage of tRNA, making it much more resistant to hydrolysis and halting the activity of the ribosome.3 These properties of puromycin have been exploited in assays for peptide-bond formation and elongation and also the antibiotic is often used as selective agent in cell culture systems. |
| 别名 | CL 13,900;CL 16,536;NSC 3055;PDH; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Azzam, M.E. and Algranati, I.D. Mechanism of puromycin action: Fate of ribosomes after release of nascent protein chains from polysomes. Proc.Nat.Acad.Sci.USA 70(12), 3866-3869 (1973).
2.Rodriguez-Fonseca, C.,Phan, H.,Long, K.S., et al. Puromycin-rRNA interaction sites at the peptidyl transferase center. RNA 6(5), 744-754 (2000).
3.Lührmann, R.,Bald, R.,Stöffler-Meilicke, M., et al. Localization of the puromycin binding site on the large ribosomal subunit of Escherichia coli by immunoelectron microscopy. Proceedings of the National Academy of Sciences of the United States of America 78(12), 7276-7280 (1981).
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| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 544.4 |
| 分子式 | C22H29N7O5 • 2HCl |
| CAS号 | 58-58-2 |
| 稳定性 | ≥ 2 years |
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