| 货号 | 17601-5mg |
| 描述 | Proteinase 3 (PR3, myeloblastin) is a polymorphonuclear leukocyte serine proteinase that degrades matrix proteins including fibronectin, laminin, vitronectin, and collagen type IV to generate antimicrobial peptides.1 Neutrophil elastase is a serine proteinase that is secreted by neutrophils during inflammation to destroy pathogens.2 Evaluating these enzymes is helpful to understanding inflammatory autoimmune processes. MeOSuc-AAPV-pNA is a highly sensitive peptide substrate that is hydrolyzed by both human and mouse neutrophil elastase and PR3, but not cathepsin G or chymotrypsin.3,4 Enzyme activity can be quantified by colorimetric detection of free p-nitroanilide at 405 nm. |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Rao, N.V.,Wehner, N.G.,Marshall, B.C., et al. Characterization of proteinase-3 (PR-3), a neutrophil serine proteinase. Structural and functional properties. The Journal of Biological Chemisty 266(15), 9540-9548 (1991). 2.Mantovani, A.,Cassatella, M.A.,Costantini, C., et al. Neutrophils in the activation and regulation of innate and adaptive immunity. Nature Reviews.Immunology 11(8), 519-531 (2011). 3.Wiesner, O.,Litwiller, R.D.,Hummel, A.M., et al. Differences between human proteinase 3 and neutrophil elastase and their murine homologues are relevant for murine model experiments. FEBS Letters 579(24), 5305-5312 (2005). 4.Korkmaz, B.,Attucci, S.,Hazouard, E., et al. Discriminating between the activities of human neutrophil elastase and proteinase 3 using serpin-derived fluorogenic substrates. The Journal of Biological Chemisty 277(42), 39074-39081 (2002). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 590.6 |
| 分子式 | C27H38N6O9 |
| CAS号 | 70967-90-7 |
| 稳定性 | ≥ 2 years |
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