| 货号 | 17341-250mg |
| 描述 | Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative.1 It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease.2,3 NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions.4 More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity.5 Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate. |
| 别名 | NBT;p-Nitro Blue Tetrazolium;Nitrotetrazolium Blue;NSC 27622; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010). 2.Freeman, R., and King, B. Technique for the performance of the nitro-blue tetrazolium (NBT) test. Journal of Clinical Pathology 25(10), 912-914 (1972). 3.Nathan, D.G.,Baechner, R.L., and Weaver, D.K. Failure of nitro blue tetrazolium reduction in the phagocytic vacuoles of leukocytes in chronic granulomatous disease. Journal of Clinical Investigation 48(10), 1895-1904 (1969). 4.Nisimoto, Y.,Wilson, E.,Heyl, B.L., et al. NADH dehydrogenase from bovine neutrophil membranes. Purification and properties. The Journal of Biological Chemisty 261(1), 285-290 (1986). 5.Trinh, L.A.,McCutchen, M.D.,Bonner-Fraser, M., et al. Fluorescent in situ hybridization employing the conventional NBT/BCIP chromogenic stain. Biotechniques 42(6), 756-759 (2007). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 817.6 |
| 分子式 | C40H30N10O6 • 2Cl |
| CAS号 | 298-83-9 |
| 稳定性 | ≥ 2 years |
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