| 货号 | 16459-5mg |
| 描述 | Biotin-HPDP is a sulfhydryl-reactive biotinylation reagent that forms a reversible disulfide linkage. It is used to label protein cysteines and other substrates that contain sulfhydryl groups.1,2,3 Biotin-HPDP is also used in the biotin switch technique to tag S-nitrosylated (SNO) proteins, following reduction of SNO groups to thiols.4,5 Compounds that are tagged with biotin interact avidly with streptavidin-coupled beads, fluorophores, enzymes, etc. The interaction of biotin-HPDP with substrates containing sulfhydryl groups is easily performed at pH 6.5 to 7.5 in buffers such as PBS. The disulfide linkage that is formed between avidin and substrate can later by cleaved by a reducing agent, like dithiothreitol. |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Lu, C.,Kavalier, A.,Lukyanov, E., et al. S-sulfhydration/desulfhydration and S-nitrosylation/denitrosylation: A common paradigm for gasotransmitter signaling by H2S and NO. Methods: A Companion to Methods in Enzymology 62(2), 177-181 (2013). 2.Slatin, S.L.,Nardi, A.,Jakes, K.S., et al. Translocation of a functional protein by a voltage-dependent ion channel. Proceedings of the National Academy of Sciences of the United States of America 99(3), 1286-1291 (2002). 3.Bizzozero, O.A. and Zheng, J. Identification of major S-nitrosylated proteins in murine experimental autoimmune encephalomyelitis. Journal of Neuroscience Research 87(13), 2881-2889 (2009). 4.Ckless, K.,Reynaert, N.L.,Taatjes, D.J., et al. In situ detection and visualization of S-nitrosylated proteins following chemical derivatization: Identification of Ran GTPase as a target for S-nitrosylation. Nitric Oxide: Biology and Chemistry 11, 216-227 (2004). 5.Forrester, M.T.,Foster, M.W. and Stamler, J.S. Assessment and application of the biotin switch technique for examining protein S-Nitrosylation under conditions of pharmacologically induced oxidative stress. The Journal of Biological Chemisty 282(19), 13977-13983 (2007). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥95% |
| 计算分子量 | 539.8 |
| 分子式 | C24H37N5O4S3 |
| CAS号 | 129179-83-5 |
| 稳定性 | ≥ 2 years |
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