| 货号 | 15926-10mg |
| 描述 | 5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) is a redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms.1,2,3 This membrane-permeable indicator is readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm).3,4 While the oxidized salt is non-fluorescent, the reduced formazan can be evaluated using a standard rhodamine filter set (excitation: 510-560 nm; emission > 590 nm), although optimum excitation requires a blue 420 nm excitation filter.1,2 For flow cytometry, a 480 nm excitation laser combined with detection in the red region is suitable. |
| 别名 | CTC;Cyanotolyl Tetrazolium Chloride; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Rodriguez, G.G.,Phipps, D.,Ishiguro, K., et al. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Applied and Environmental Microbiology 58(6), 1801-1808 (1992). 2.Caruso, G.,Mancuso, M. and Crisafi, E. Combined fluorescent antibody assay and viability staining for the assessment of the physiological states of Escherichia coli in seawaters. Journal of Applied Microbiology 95(2), 225-233 (2003). 3.Sieracki, M.E.,Cucci, T.L. and Nicinski, J. Flow cytometric analysis of 5-cyano-2,3-ditolyl tetrazolium chloride activity of marine bacterioplankton in dilution cultures. Applied and Environmental Microbiology 65(6), 2409-2417 (1999). 4.Bhupathiraju, V.K.,Hernandez, M.,Landfear, D., et al. Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria. J.Microbiol.Methods 37(3), 231-243 (1999). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 计算分子量 | 311.8 |
| 分子式 | C16H14N5 • Cl |
| CAS号 | 90217-02-0 |
| 稳定性 | ≥ 2 years |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |