| 货号 | 14872-50mg |
| 描述 | Lucigenin is a dual purpose probe. It can be used as a chemiluminescent probe to detect superoxide production by enzymatic and cellular sources.1,2,3 It is a very sensitive method that has been applied to the monitoring of superoxide production from xanthine oxidase, microsomal NADPH cytochrome reductase, and NADPH oxidases of phagocytes, endothelial cells, fibroblasts, and smooth muscle cells of blood vessel walls.2,4 Lucigenin is also used as a fluorescent chloride-sensitive indicator, with fluorescence being quenched by chloride (excitation: 455 nm; emission: 505 nm).5,6,7 Lucigenin fluorescence is insensitive to phosphate, sulfate, and nitrate.5 |
| 别名 | L-6,868;NSC 151912; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Li, Y.,Zhu, H.,Kuppusamy, P., et al. Validation of lucigenin (Bis-N-methylacridinium) as a chemilumigenic probe for detecting superoxide anion radical production by enzymatic and cellular systems. The Journal of Biological Chemisty 273(4), 2015-2023 (1998). 2.Vladimirov, Y.A. and Proskurnina, E.V. Free radicals and cell chemiluminescence. Biochemistry.Biokhimiia 74(13), 1545-1566 (2009). 3.Skatchkov, M.P.,Sperling, D.,Hink, U., et al. Validation of lucigenin as a chemiluminescent probe to monitor vascular superoxide as well as basal vascular nitric oxide production. Biochemical and Biophysical Research Communications 254, 319-324 (1999). 4.Cai, H.,Dikalov, S.,Griendling, K.K., et al. Detection of reactive oxygen species and nitric oxide in vascular cells and tissues: Comparison of sensitivity and specificity. Vascular Biology Protocols 139, 293-311 (2007). 5.Biwersi, J.,Tulk, B. and Werkman, A.S. Long-wavelength chloride-sensitive fluorescent indicators. Analytical Biochemistry 219(1), 139-143 (1994). 6.Ferdani, R.,Li, R.,Pajewski, R., et al. Transport of chloride and carboxyfluorescein through phospholipid vesicle membranes by heptapeptide amphiphiles. Organic & Biomolecular Chemistry 5(15), 2423-2432 (2007). 7.Ruedas-Rama, M.J.,Orte, A.,Hall, E.A.H., et al. A chloride ion nanosensor for time-resolved fluorimetry and fluorescence lifetime imaging. Analyst 137(6), 1500-1508 (2012). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥95% |
| 计算分子量 | 510.5 |
| 分子式 | C28H22N2 • 2NO3 |
| CAS号 | 2315-97-1 |
| 稳定性 | ≥ 2 years |
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