| 货号 | 13242-25mg |
| 描述 | Autophagy is a multi-step process that involves the degradation and digestion of intracellular components by the lysosome. This process allows cells to efficiently mobilize and recycle cellular constituents, and also prevents the accumulation of damaged organelles, misfolded proteins, and invading microorganisms. mTOR, whose activation is controlled by phosphoinositide 3-kinase (PI3K), is a key regulator of autophagy.1 3-Methyladenine (3-MA) is a specific inhibitor of PI3K activity and one of the most widely used inhibitors of the initial phase of the autophagic process: the sequestering of cytoplasmic material by the lysosome.2,3 At 5 mM, 3-MA inhibits protein degradation in rat hepatocytes by 65%.2 3-MA has been shown to block class I, class II, and class III PI3Ks, including some downstream targets, and to suppress the invasion of highly metastatic human fibrosarcoma HT1080 cells at 10 mM.4,5 |
| 别名 | 3-MA;NSC 66389; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Backer, J.M. The regulation and function of class III PI3Ks: Novel roles for Vps34. Biochemistry Journal 410, 1-17 (2008). 2.Seglen, P.O., and Gordon, P.B. 3-Methyladenine: Specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proceedings of the National Academy of Sciences of the United States of America 79, 1889-1892 (1982). 3.Chang, Y.Y.,Juhász, G.,Goraksha-Hicks, P., et al. Nutrient-dependent regulation of autophagy through the target of rapamycin pathway. Biochemical Society Transactions 37, 232-236 (2009). 4.Wu, Y.T.,Tan, H.L.,Shui, G., et al. Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. The Journal of Biological Chemisty 285(14), 10850-10861 (2010). 5.Ito, S.,Koshikawa, N.,Mochizuki, S., et al. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. International Journal of Oncology 31, 261-268 (2007). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥95% |
| 计算分子量 | 149.2 |
| 分子式 | C6H7N5 |
| CAS号 | 5142-23-4 |
| 稳定性 | ≥ 2 years |
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