| 货号 | 10009542-10mg |
| 描述 | The tetracycline repressor (TetR) is a transcriptional regulator which normally binds tightly to its palindromic tetO operator DNA, blocking gene expression.1 Tet causes the repressor to dissociate from the DNA, allowing transcription to occur. A novel reverse TetR (revTetR) requires tetracycline as a co-repressor to bind tetO and block transcription.2,3 Anhydrotetracycline (hydrochloride) is a powerful effector in both the tetracycline repressor (TetR) and reverse TetR (revTetR) systems, binding the Tet repressor 35-fold more strongly than Tet.1,4 Moreover, anhydrotetracycline poorly binds the 30S ribosomal subunit, compared to Tet,5 so it does not act as a general inhibitor of translation and is a poor antibiotic. Perhaps related to this, the concentration of anhydrotetracycline that inhibits eukaryotic cell growth is more than a 1,000-fold above the dose that alters transcription through TetR.1 |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Gossen, M., and Bujard, H. Anhydrotetracycline, a novel effector for tetracycline controlled gene expression systems in eukaryotic cells. Nucleic Acids Research 21(18), 4411-4412 (1993). 2.Kamionka, A.,Bogdanska-Urbaniak, J.,Scholz, O., et al. Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor. Nucleic Acids Research 32(2), 842-847 (2004). 3.Resch, M.,Striegl, H.,Henssler, E.M., et al. A protein functional leap: How a single mutation reverses the function of the transcription regulator TetR. Nucleic Acids Research 36(13), 4390-4401 (2008). 4.Degenkolb, J.,Takahashi, M.,Ellestad, G.A., et al. Structural requirements of tetracycline-Tet repressor interaction: Determination of equilibrium binding constants for tetracycline analogs with the Tet repressor. Antimicrobial Agents and Chemotherapy 35(8), 1591-1595 (1991). 5.Rasmussen, B.,Noller, H.F.,Daubresse, G., et al. Molecular basis of tetracycline action: Identification of analogs whose primary target is not the bacterial ribosome. Antimicrobial Agents and Chemotherapy 35(11), 2306-2311 (1991). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 462.9 |
| 分子式 | C22H22N2O7 • HCl |
| CAS号 | 13803-65-1 |
| 稳定性 | ≥ 2 years |
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