| 货号 | 12013-5mg |
| 描述 | Dihydroethidium is a cell-permeable blue fluorescent dye that upon entering cells interacts with superoxide to form oxyethidium, which intercalates with nucleic acids and emits a red fluorescence detectable qualitatively by fluorescent microscopy or quantitatively by HPLC.1 This selective and sensitive probe has been used to detect reactive oxygen species during the phagocytic respiratory burst and for the detection of intracellular superoxide in cultured cells.2,3,4 |
| 别名 | Hydroethidine;PD-MY 003; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Cai, H.,Dikalov, S.,Griendling, K.K., et al. Detection of reactive oxygen species and nitric oxide in vascular cells and tissues: Comparison of sensitivity and specificity. Vascular Biology Protocols 139, 293-311 (2007). 2.Fink, B.,Laude, K.,McCann, L., et al. Detection of intracellular superoxide formation in endothelial cells and intact tissues using dihydroethidium and an HPLC-based assay. Am. J. Physiol. Cell Physiol. 287, C895-C902 (2004). 3.Robinson, K.M.,Janes, M.S.,Pehar, M., et al. Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proceedings of the National Academy of Sciences of the United States of America 103(41), 15038-15043 (2006). 4.Salih, H.R.,Husfeld, L., and Adam, D. Simultaneous cytofluorometric measurement of phagocytosis, burst production and killing of human phagocytes using Candida albicans and Staphylococcus aureus as target organisms. Clin.Microbiol.Infect. 6(5), 251-258 (2000). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 315.4 |
| 分子式 | C21H21N3 |
| CAS号 | 104821-25-2 |
| 稳定性 | ≥ 2 years |
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