| 货号 | 9400-SO-050 |
| 别名 | Stanniocalcin 1; stanniocalcin-1; STC1; STC-1; STCSTC-1 |
| 来源 | Mouse myeloma cell line, NS0-derived Thr18-Ala247, with a C-terminal 6-His tag |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6781 (Human) |
| 内毒素水平 | <0.10 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 20-120 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN) . |
| 溶解方法 | Reconstitute at 500 μg/mL in PBS. |
| 背景 | Stanniocalcin 1 (STC-1) is a homodimeric glycoprotein hormone that isinvolved in calcium and phosphate homeostasis. It was originally identified in bony fishes, where elevation of calciumin serum causes the release of STC from the endocrine glands called the corpusclesof Stannius (1-3). In contrast to fishwhere expression is mainly in the corpuscles of Stannius, the STC-1 gene inmammals is widely expressed in tissues including thyroid, ovary, prostate, adrenalgland, muscle, intestine, kidney, heart and lung (4). Human STC-1 protein consists of a 16 amino acid (aa)propeptide region and a 214 aa STC-1 chain. The amino acid sequence of human mature STC-1 is 36%identical to that of human STC-2. STC-1 is highly homologous with mouse,sharing 95% amino acid identity. STC-1 inhibits thebreakdown of PAPP-A (5), protects cancer cells from apoptosis (4), reducestumor size of liver cancers (6), promotes osteoblast differentiation (7) and inhibitslongitudinal bone growth directly at the growth plate (8). It is also abiomarker of brain and lung cancer progression (7, 8). |
| Accession # | P52823-1 N-termina |
| 运输条件 | Blue Ice |
| 预期分子量 | 27 kDa |
| SDS-PAGE | 26-43 kDa, reducing conditions |
| 存放说明 | -20℃ |
| 纯度 | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| 参考文献 |
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