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货号： bs-1542R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1542R
- 英文名称
- CCR6
- 中文名称
- 细胞表面趋化因子受体6抗体
- 别 名
- CKR6; C-C chemokine receptor type 6; BN 1; BN1; C C chemokine receptor type 6; C C CKR 6; CC chemokine receptor type 6; CC CKR 6; CC R6; CCCKR6; CCR 6; CD 196; CD196; CD196 antigen; Chemokine (C C motif) receptor 6; Chemokine (C C) receptor 6; Chemokine (CC motif) receptor 6; Chemokine (CC) receptor 6; Chemokine receptor 6; Chemokine receptor like 3; CKR 6; CKR L3; CKRL3; CMKBR 6; CMKBR6; DCR 2; DCR2; DRY 6; DRY6; G protein coupled receptor 29; GPR 29; GPR CY4; GPR29; GPRCY 4; GPRCY4; LARC receptor; Seven transmembrane receptor lymphocyte 22; STRL 22; STRL22; CCR6_HUMAN.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 信号转导 转录调节因子 细胞表面分子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg /test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 41kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CCR6:201-300/374 <Cytoplasmic>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein coupled receptors. It is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP 3 alpha). This receptor has been shown to be important for B lineage maturation and antigen driven B cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses.CCR6 has been reported to be expressed in intestine, colon, liver, lymph node, spleen, lung, blood, umbilical cord, placenta, skin, and pancreas. It has also been reported to be expressed in lymphocytes, T cells, and B cells.
Function:
Receptor for a C-C type chemokine. Binds to MIP-3-alpha/LARC and subsequently transduces a signal by increasing the intracellular calcium ions level.
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Tissue Specificity:
Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
P51684
Gene ID:
1235
Database links:Entrez Gene: 1235 Human
Entrez Gene: 12458 Mouse
Entrez Gene: 308163 Rat
Omim: 601835 Human
SwissProt: P51684 Human
SwissProt: O54689 Mouse
Unigene: 46468 Human
Unigene: 8007 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CCR6/CD196也是是属于G蛋白偶联受体一家族成员，参与调节白细胞的迁移和活性作用，是细胞膜蛋白。
- 产品图片
Blank control: Raji(blue).
Primary Antibody:Rabbit Anti- CCR6 antibody(bs-1542R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-1542R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.