产品介绍:
bisBenzimide H 33342 trihydrochloride ≥95% (TLC)
Synonym
2’-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole trihydrochloride
bisBenzimide
HOE 33342
Hoechst 33342
Molecular FormulaC27H28N6O ・ 3HCl ・ xH2O
Molecular Weight 561.93 (anhydrous basis)
CAS Number 23491-52-3
Descriptions
Application Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
Biochem/physiol Actions Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.
Properties
Assay……………………………….. ≥95% (TLC)
solubility H2O: 20 mg/mL
phosphate buffers: precipitates
storage temp. −20°C
TEST | SPECIFICATION |
APPEARANCE | YELLOW TO GREEN POWDER |
SOLUBILITY | CLEAR TO VERY SLIGHTLY HAZY YELLOW TO BROWN SOLUTION AT 50MG PLUS 1ML OF WATER |
IDENTITY | CONSISTENT WITH STRUCTURE BY IR OR NMR |
WATER BY KARL FISCHER | NMT 16.1% (6MOLES/MOLE) |
ELEMENTAL ANALYSIS * | 54.8 TO 60.6% CARBON |
14.2 TO 15.7% NITROGEN | |
PURITY BY THIN LAYER CHROMATOGRAPHY | NLT 95% |
RECOMMENDED RETEST | 3 YEARS |
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