细胞名称

A549人非小细胞肺癌细胞

描述

该细胞系由D.J.Griad通过肺癌组织移植培养建系，患者为58岁白人男性。 A549能通过胞苷二磷酸胆碱途径合成含有高含量不饱和脂肪酸的卵磷脂。

动物种别

人

性别

男

组织来源

肺癌

形态

上皮细胞

培养基和添加剂

F12K培养基(SIGMA,货号N3520，添加NaHCO3 2.5g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

REFERENCE

Giard DJ , et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 Mayr GA , Freimuth P . A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365 Goodrum FD , Ornelles DA . The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383 St. Geme JW , et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830 Horikami SM , et al. The Sendai virus V protein interacts with the NP protein to regulate viral genome RNA replication. Virology 222: 383-390, 1996. PubMed: 8806522 Huang S , et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475 Goodrum FD , et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 Fang R , Aust AE . Induction of ferritin synthesis in human lung epithelial cells treated with crocidolite asbestos. Arch. Biochem. Biophys. 340: 369-375, 1997. PubMed: 9143343 Geiger T , et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 Evdokiou A , Cowled PA . Tumor-suppressive activity of the growth arrest-specific gene GAS1 in human tumor cell lines. Int. J. Cancer 75: 568-577, 1998. PubMed: 9466658 Giavedoni LD , Yilma T . Construction and characterization of replication-competent simian immunodeficiency virus vectors that express gamma interferon. J. Virol. 70: 2247-2251, 1996. PubMed: 8642649 Bartz SR , et al. Human immunodeficiency virus type 1 cell cycle control: Vpr is cytostatic and mediates G2 accumulation by a mechanism which differs from DNA damage checkpoint control. J. Virol. 70: 2324-2331, 1996. PubMed: 8642659 Garofalo R , et al. Transcriptional activation of the interleukin-8 gene by respiratory syncytial virus infection in alveolar epithelial cells: nuclear translocation of the RelA transcription factor as a mechanism producing airway mucosal inflammation. J. Virol. 70: 8773-8781, 1996. PubMed: 8971006 Jamaluddin M , et al. Inducible translational regulation of the NF-IL6 transcription factor by respiratory syncytial virus infection in pulmonary epithelial cells. J. Virol. 70: 1554-1563, 1996. PubMed: 8627674 Lewis JA , et al. Inhibition of mitochondrial function by interferon. J. Biol. Chem. 271: 13184-13190, 1996. PubMed: 8662694 Lieber M , et al. A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int. J. Cancer 17: 62-70, 1976. PubMed: 175022 Acta Cell Biology Sinica 24(6):379-382,2002 Acta Biochimica et Biophysica Sinica 34(6):748-752, 2002 Chin J Cell Mol Immunol 18(4):332-334,2002 CHINA ONCOLOGY 12(5):395-398,2002 CHINA ONCOLOGY 12(5):441-443,2002 Chin J Oncol. 24(6):537-539,2002 Acta Biochimica et Biophysica Sinica 35(6):548-553,2003 Tumor 23(2):140-142,2003 Chin J Microbiol Immunol 23(4):288-291,2003 Chinese Journal of Cancer 22(2):171-174,2003 Suzhou University Journal of Medical Science 23(3):284-285,2003 The FASEB Journal 16:294,2002